牦牛新鲜囊胚与玻璃化冻融囊胚转录组的比较分析

doi:10.11843/j.issn.0366-6964.2018.04.007

Abstract

Abstract:

The study aimed to investigate the transcriptome differential expression between pre-and post-vitrified yak blastocysts in order to understand the effect of vitrification on the gene expression profile of yak blastocysts. Total RNAs were extracted respectively from fresh blastocysts (FRB) and vitrified-thawed blastocysts (VTB) that were in vitro-produced from yaks, and then the Smart-seq2 method was used to amplify and construct RNA libraries, and finally transcriptome was sequenced using RNA-seq. The|log₂ (VTB/FRB)| ≥ 1 and Q-value<0.05 were set as thresholds for identifying differentially expressed gene (DEG). The DEGs were then searched against the GO and KEGG database for enrichment analysis. The results showed that 9 827 and 13 567 transcripts were detected in FRB and VTB, respectively. A total of 11 174 DEGs were identified between the 2 libraries, in which 7 037 were up-regulated, and 4 137 were down-regulated in VTB. 10 538 DEGs were significantly enriched in 479 GO terms (P<0.05), and there were 318 pathways involved, of which 8 pathways including ribosome biogenesis in eukaryotes, spliceosome and neuroactive ligand-receptor interaction were significantly enriched (P<0.05). In conclusion, this report analyzed the transcriptome change pre-and post-vitrification of yak blastocysts using RNA-seq, which provides a theoretical basis to explore the mechanism of vitrification damage and improve vitrification technology of yak blastocysts.

CLC Number:

S823.8⁺5.2

PU Si-ying, ZHENG Jie, YANG Yuan-xiao, WANG Qin, YANG Rao-fen, ZI Xiang-dong. Comparative Transcriptome Analysis between Fresh and Vitrified-thawed Blastocysts of the Yak (Bos grunniens)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 709-717.

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Comparative Transcriptome Analysis between Fresh and Vitrified-thawed Blastocysts of the Yak (Bos grunniens)

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