SOX5影响小鼠皮肤黑色素细胞MITF-M表达和黑色素生成

doi:10.11843/j.issn.0366-6964.2018.01.010

Abstract

Abstract:

To investigate the function of SOX5 in mouse skins and the regulation of hair color formation, the differential expression of SOX5 in skins of different coat color mouse skin was examined. In this study, C57BL/6 strains of black, brown and gray mice were selected each of the 3 for 12 days after birth, randomly. Quantitative real-time PCR (qRT-PCR), Western blotting and immunohistochemistry methods were used in this study to analyze the expression of SOX5; we also constructed the eukaryotic expression vector of SOX5 and transfected the mouse melanocytes to detect the expression level of the pigmentation-related genes, and measured the melanin content. The results showed that:1) SOX5 could be expressed in mouse skin with different hair color. The mRNA and protein expression levels of SOX5 in black and gray mouse skin were higher than that in brown mouse skin with very significantly difference (P<0.01). And the main expression site of SOX5 was hair follicle outer root sheath. 2) Compared with empty vector group(Vector-GFP), the mRNA and protein expression levels of MITF-M,TYR, TYRP1,TYRP2,PMEL and OA1 were increased with significant difference (P<0.05) and very significantly difference (P<0.01) in SOX5 transfected group; the content of melanin was significantly increased with very significantly difference (P<0.01) in SOX5 transfected group. 3) MITF-M had a negative feedback on SOX5 by overexpression of MITF-M.These suggest that SOX5 affect the formation of pigments and then involve in the formation of coat color by regulating MITF-M.

CLC Number:

Q343

CHANG Lu-cheng, ZHAO Bing-ling, LIU Ying, BAI Yun, LI Jing-wei, LI Li-xin, WANG Hai-dong. Effects of SOX5 on MITF-M Expression and Melanogenesis in Mouse Skin Melanocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 83-91.

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Effects of SOX5 on MITF-M Expression and Melanogenesis in Mouse Skin Melanocytes

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