ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2017, Vol. 48 ›› Issue (9): 1611-1623.doi: 10.11843/j.issn.0366-6964.2017.09.006

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Identification of RNA Editing Sites in Chicken Lines Divergently Selected for Abdominal Fat Content

LIANG Hao, LI Min, DONG Xiang-yu, LI Hui, DU Zhi-qiang*   

  1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, Key Laboratory of Chicken Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
  • Received:2017-03-28 Online:2017-09-23 Published:2017-09-23

Abstract:

This study aimed to obtain the RNA editing sites information in adipose tissue in chicken lines divergently selected for abdominal fat content, and provide the foundation for fat study of broiler. Ten individuals from the 19th generation of fat and lean broiler lines divergently selected for abdominal fat content from the Northeast Agricultural University were used. The transcriptome and genome sequencing datasets were used for identifying RNA editing sites in abdominal fat tissues. Bioinformatics method was used to construct the candidate set of RNA editing sites, after stringent data filtering and screening. The result showed that we classified and annotated RNA editing sites in the candidate set.In the adipose tissue of lean line broilers, 28 transition, 101 transversion and 71 insertion-deletion (InDel) RNA editing sites types were found; while in the adipose tissue of fat line broilers, 30 transition, 84 transversion and 77 InDel RNA editing sites types were found. Most of RNA editing sites were located in the regulatory and intergenic regions of genes (intron 41.91%; intergenic 33.08%; downstream 20.59%; upstream 2.94% and exon 1.47%, respectively). We validated 4 RNA editing sites, 3 of which located in genes (ATP1B3, SLC6A6 and FLNB) related to chicken adipogenesis. In the current study, identification and validation of RNA editing sites were performed in chicken abdominal fat tissues, which lays the foundation for further investigating the molecular mechanism of RNA editing in adipose tissue growth and development.

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