Abstract:

In order to clone, sequence bioinformatic analysis and expression pattern determination of CYP19A1 gene in swamp buffalo, a pair of special primers were designed according to released sequence of bovine CYP19A1 in GenBank. The CYP19A1 gene was amplified by RT-PCR, its gene sequence character and protein structure was systemically analysised by bioinformatics techniques. The expression patterns of buffalo CYP19A1 in different tissues were also assayed with qRT-PCR. The results showed that the cloned 1 587 bp buffalo CYP19A1 gene fragment including a 1 512 bp whole length CDS (coded 503 amino acids). The sequence multialigned results showed that buffalo CYP19A1 gene shared 99%, 98%, 91%, 86% and 84% of similar nucleotide sequence with that of Bos taurus, Ovis aries, Sus scrofa and Homo sapiens, respectively. In addition, the expression pattern analysis results showed that buffalo CYP19A1 expressed in all the seven detected tissues, with the most abundant expression in brain and pituitary gland, followed by skeleton and ovary, the minimal expression in muscle was observed. The cloning and analysis of CYP19A1 gene provided an important foundation for further study regulation mechanism of CYP19A1 gene in buffalo in the future.