小尾寒羊间情期和发情期microRNAs差异表达分析

doi:10.11843/j.issn.0366-6964.2016.07.004

Abstract

Abstract:

To explore the theoretical basis of the microRNAs in regulating the reproductive process in Small Tail Han sheep，the ovarian microRNAs profiles were constructed and analyzed in estrus and diestrus.One ovary was collected with surgery method at estrus (the 1st day) and diestrus (the 13th day) from the same sheep，respectively.The RNA sequencing data were obtained with illumina Hiseq2000 sequencing platform.The expression profiles and differential expression of microRNAs were analyzed by bioinformatics method.The real-time quantitative PCR (qRT-PCR) was used for validating the differentially expressed microRNAs between the 2 periods in ovary.The microRNAs expression profiles of Small Tailed Han sheep was constructed in the period of estrus and diestrus in ovaries.The highest expression of microRNA was observed for oar-miR-99a and oar-miR-143 in the period of diestrus and estrus，respectively.Three microRNAs，oar-miR-200a，oar-miR-200b and oar-miR-200c，were significantly differentially expressed between estrus and diestrus.The expression level of 2 randomly selected microRNAs by qRT-PCR was the same as the result by RNA-seq data.These microRNAs expression profiles will be used to provide informations for further studying the microRNAs in sheep ovary.The differentially expressed microRNAs may regulate the ovarian periodic activity through the metabolism and immunity pathway by the analysis of predicted target genes and enriched pathways.

Key words: Small Tailed Han sheep, diestrus, estrus, ovary, microRNA

CLC Number:

S826
S813.3

DUAN Xin-chong，WEI Yan-hui，LI Yang，LI Xiang-yun，ZHOU Rong-yan，XI Jian-zhong. Analyzing the Differential Expression of microRNAs in Estrus and Diestrus of Small Tailed Han Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1324-1332.

References

［1］HU S J，REN G，LIU J L，et al.MicroRNA expression and regulation in mouse uterus during embryo implantation［J］.J Biol Chem，2008，283(34)：23473-23484.
［2］CARLETTI M Z，CHRISTENSON L K.MicroRNA in the ovary and female reproductive tract［J］.J Anim Sci，2009，87(14)：29-38.
［3］徐盛玉，王定越，吴德.MicroRNA及其对哺乳动物繁殖的影响［J］.畜牧兽医学报，2011，42（6）：747-753.
XU S Y，WANG D Y，WU D.Effects of MicroRNA on the Mammalian Reproduction［J］.Acta Veterinaria et Zootechnica Sinica，2011，42（6）：747-753.（in Chinese）
［4］CHRISTENSON L K.MicroRNA control of ovarian function［J］.Anim Reprod Sci， 2010，7(3)：129-133.
［5］SIROTKIN A V，OVCHARENKO D，GROSSMANN R，et al.Identification of microRNAs controlling human ovarian cell steroidogenesis via a genome-scale screen［J］. J Cell Physiol，2009，219(2)：415-420.
［6］XU S，LINHER-MELVILLE K，YANG B B，et al.Micro-RNA378 (miR-378) regulates ovarian estradiol production by targeting aromatase［J］.Endocrinology，2011，152(10)：3941-3951.
［7］YAN G J，ZHANG L，FANG T，et al.MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB［J］. FEBS Lett，2012，586(19)：3263-3270.
［8］DAVIS B N，HILYARD A C，LAGNA G，et al.SMAD proteins control DROSHA-mediated microRNA maturation［J］.Nature， 2008，454(7200)：56-61.
［9］VO N，KLEIN M E，VARLAMOVA O，et al.A cAMP-response element binding protein-induced microRNA regulates neuronal morphogenesis［J］.Proc Natl Acad Sci U S A，2005，102(45)：16426-16431.
［10］MURAMATSU F，KIDOYA H，NAITO H，et al.microRNA-125b inhibits tube formation of blood vessels through translational suppression of VE-cadherin［J］.Oncogene，2013，32(4)：414-421.
［11］DI R，HE J，SONG S，et al.Characterization and comparative profiling of ovarian microRNAs during ovine anestrus and the breeding season［J］.BMC Genomics，2014，15(1)：899.
［12］WEN M，SHEN Y，SHI S，et al.miREvo：An integrative microRNA evolutionary analysis platform for next-generation sequencing experiments［J］.BMC Bioinformatics，2012，13(1)：140.
［13］FRIEDLANDER M R，MACKOWIAK S D，LI N，et al.miRDeep2 accurately identifies known and hundreds of novel microRNA genes in seven animal clades［J］.Nucleic Acids Res，2011，40(1)：37-52.
［14］MIAO X Y，LUO Q M，QIN X Y.Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity［J］.Mol Cell Endocrinol，2015，15(402)：32-42.
［15］BARTEL D P.MicroRNAs：target recognition and regulatory functions［J］.Cell，2009，136(2)：215-233.
［16］VALEGGIA C，EILLSON P T.Interactions between metabolic and reproductive functions in the resumption of postpartum fecundity［J］.Am J Hum Biol，2009，21(4)：559-566.
［17］HOSSAIN M M，GHANEM N，HOELKER M，et al.Identification and characterization of miRNAs expressed in the bovine ovary［J］.BMC Genomics，2009，10(1)：443.
［18］LI M Z，LIU Y K，WANG T，et al.Repertoire of porcine microRNAs in adult ovary and testis by deep sequencing［J］.Int J Biol Sci， 2011，7(7)：1045-1055.
［19］MISHIMA T，TAKIZAWA T，LUO S S，et al.MicroRNA (miRNA) cloning analysis reveals sex differences in miRNA expression profiles［J］.Reproduction，2008，136(6)：811-822.
［20］CARLETTI M Z，FIEDLER S D，CHRISTENSON L K.MicroRNA 21 blocks apoptosis in mouse periovulatory granulosa cells［J］.Biol Reprod，2010，83(2)：286-295.
［21］ZHANG J F，JI X W，ZHOU D D，et al.miR-143 is critical for the formation of primordial follicles in mice［J］.Front Biosci，2012(1)，18：588-597.
［22］OTSUKA M，ZHENG M，HAYASHI M，et al.Impaired microRNA processing causes corpus luteum insufficiency and infertility in mice［J］.J Clin Invest，2008，118(5)：1944-1954.
［23］TRIPURANI S K，XIAO C，SALEM M，et al.Cloning and analysis of fetal ovary microRNAs in cattle［J］.Anim Reprod Sci，2010，120(1)：16-22.
［24］ROUSH S，SLACK F J.The let-7 family of microRNAs［J］.Trends Cell Biol，2008，18(10)：505-516.
［25］MAKRIGIANNAKIS A，COUKOS G，CHRISTOFIDOU-SOLOMIDOU M，et al.N-cadherin-mediated human granulosa cell adhesion prevents apoptosis：a role in follicular atresia and luteolysis?［J］.Am J Pathol，1999，154(5)：1391-1406.
［26］MORA J M，FENWICK M A，CASTLE L，et al.Characterization and significance of adhesion and junction-related proteins in mouse ovarian follicles［J］.Biol Reprod，2012，86(5)：153.
［27］AMBEKER A S，NLIRUJOG R S，SRIKANTH S M，et al.Proteomic analysis of human follicular fluid：a new perspective towards understanding folliculogenesis［J］. J Proteomics，2013，87(7)：68-77.
［28］EBISCH I M W，THOMAS C M G，WETZELS A M M，et al.Review of the role of the plasminogen activator system and vascular endothelial growth factor in subfertility［J］. Fertil Steril，2008，90(6)：2340-2350.
［29］WANG C，ROY S K.Gonadotropins and steroids regulation of ZEB1 expression in the hamster ovary［J］.Biol Reprod，2011，85(1 Supplement)：661.
［30］HASUWA H，UEDA J，IKAWA M，et al.MiR-200b and miR-429 Function in mouse ovulation and are essential for female fertility［J］.Science，2013，341(6141)：71-73.
［31］任红艳，刘楠，陶聪，等.小鼠体型控制相关microRNA-200b(miR-200b)的表达谱及靶标分析［J］.农业生物技术，2013，21(1)：47-54.
REN H Y，LIU N，TAO C，et al.The expression profile and target gene analysis of mouse(Mus musculus) body size REgulation related microRNA-200b(miR-200b)［J］.Journal of Agricultural Biotechnology，2013，21(1)：47-54.(in Chinese)
［32］鲁之中，陈雪梅，曾兰，等.早孕小鼠胚胎着床前后子宫内膜microRNAs差异表达［J］.细胞生物学，2009，31(6)：848-852.
LU Z Z，CHEN X M，ZENG L，et al.The different expression of microRNAs in endometrium of early pregnant mouse during embryo implantation［J］.Chinese Journal of Cell Biology，2009，31(6)：848-852.(in Chinese)

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Analyzing the Differential Expression of microRNAs in Estrus and Diestrus of Small Tailed Han Sheep

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