利用体细胞LOH突变制备α1,3-半乳糖基转移酶基因(GGTA1)缺失的五指山小型猪

Abstract

Abstract:

The production of the α 1,3-galactosyltransferase (GGTA1) gene-deficient Wuzhishan miniature pigs will provide the valuable genetically modificated animal models for the further development of the xenotransplantation between the pig and human. Based on our previous, establishment of the fibroblast cell lines using Wuzhishan miniature pig which been knocked out single GGTA1 allele by the way of promoter-trap, then selected spontaneous loss of heterozygosity cells from fibroblast cultures of heterozygous animals using a biotin-labeled IB4 lectin attached to streptavidin-coated magnetic beads. A total of 3 122 somatic nuclear transferred embryos reconstructed with the GGTA1^-/- cells were transferred into 13 recipient pigs, four of them were pregnant, and producing 12 liveborns. Absences of GGTA1 in the cloned pigs were confirmed by PCR and Southern blotting. Flow cytometric analysis revealed that α1,3Gal antigens were not present in the cells of the cloned GGTA1^-/- pigs. The production of GGTA1^-/- pigs provided a typical paradigm and an important resource for the further development of genetically modified pigs for make organs tailored for transplantation to humans.

CLC Number:

Q812

WANG Fei,FENG Chong,LONG Chuan, YUE Cheng-he, WANG Ning, LI Xi-rui, CAO Sui-zhong, LI Ming-zhou, CHU Ming-xing, PAN Deng-ke, SHUAI Su-rong. Production of α1,3-galactosyltransferase Gene-Deficient Wuzhishan Miniature Pigs by Fibroblasts Bearing Loss of Heterozygosity Mutations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Production of α1,3-galactosyltransferase Gene-Deficient Wuzhishan Miniature Pigs by Fibroblasts Bearing Loss of Heterozygosity Mutations

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