不同来源的3株牛病毒性腹泻病毒对小鼠的致病性分析

doi:10.11843/j.issn.0366-6964.2018.10.019

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (10): 2232-2239.doi: 10.11843/j.issn.0366-6964.2018.10.019

不同来源的3株牛病毒性腹泻病毒对小鼠的致病性分析

阮文强¹, 陈新诺¹, 任玉鹏¹, 覃思楠¹, 汤承^1,2, 张斌^1,2*

1. 西南民族大学生命科学与技术学院, 成都 610041;
2. 国家民族事务委员会青藏高原动物疫病防控创新团队, 成都 610041

收稿日期:2017-12-13 出版日期:2018-10-23 发布日期:2018-10-23
通讯作者: 张斌,教授,博士,E-mail:binovy@sina.com
作者简介:阮文强(1990-),男,仡佬族,贵州铜仁人,硕士生,主要从事动物病原分子生物学研究,E-mail:810668497@qq.com
基金资助:
"十三五"国家重点研发计划（2016YFD0500907）；四川省教育厅创新团队项目（13TD0057）；西南民族大学中央高校基本科研业务费专项基金项目（2017NZYQN01）

Pathogenicity Analysis of Three Bovine Viral Diarrhea Viruses from Different Sources in Mice

RUAN Wen-qiang¹, CHEN Xin-nuo¹, REN Yu-peng¹, QIN Si-nan¹, TANG Cheng^1,2, ZHANG Bin^1,2*

1. College of Life Science Technology, Southwest Minzu University, Chengdu 610041, China;
2. Animal Disease Prevention and Control Innovation Team in the Qinghai Tibet Plateau of State Ethnic Affairs Commission, Chengdu 610041, China

Received:2017-12-13 Online:2018-10-23 Published:2018-10-23

摘要/Abstract

摘要：

为分析牛病毒性腹泻病毒（BVDV）对小鼠的致病性，选取BALB/c小鼠为试验动物，攻毒组通过腹腔注射接种不同来源的3株牦牛源BVDV1-DJ2、BVDV1-Z6和OregonC₂₄V毒株，对照组接种DMEM培养基。分别于接种后第5、7、10天收集小鼠血液和组织，并通过血常规、病理组织学、荧光定量PCR等方法对病毒感染小鼠的致病性进行研究。结果显示：攻毒后，Z6组小鼠表现的临床症状较DJ2组严重；攻毒组淋巴细胞、白细胞、血小板含量显著降低；荧光定量PCR结果显示，BVDV在肺和肝中的检出率Z6组明显高于DJ2组；HE染色可见攻毒组肝静脉内出现炎性细胞浸润、肠绒毛上皮细胞坏死和脱落，还可见脾内吞噬细胞增多和脾小结反应性增生、肺泡萎缩和出血等病理变化，与OregonC₂₄V组相比，Z6组病变最严重，DJ2组较轻。以BALB/c小鼠为试验动物，通过腹腔注射的方式可以为BVDV感染小鼠的致病性及模型的构建提供数据支持和指导。

Abstract:

This study aims at analyzing the pathogenicity of bovine viral diarrhea virus (BVDV) in mice, BALB/c mice were selected as experimental animals in this study, the challenge group were inoculated with yak-derived BVDV1-DJ2, BVDV1-Z6 and Oregon C₂₄V strains respectively by intraperitoneal injection, and the control group were inoculated with DMEM medium. The blood and tissues of the mice were collected on the 5th, 7th, and 10th day after inoculation, and the pathogenicity of the virus-infected mice were studied by blood routine, histopathology, and fluorescence quantitative PCR. The results showed, after the challenge, the clinical symptoms of the mice in the Z6 group were more severe than those in the DJ2 group. The lymphocyte, leukocyte, and platelet contents of the challenge group decreased significantly. The results of the fluorescence quantitative PCR showed that the BVDV was detected in the lung and liver. The rate of Z6 group was significantly higher than that of DJ2 group. HE staining showed that there were inflammatory cell infiltration, necrosis and shedding of intestinal villus epithelial cells in the liver of the challenge group, and it also showed that there were increased spleen endocytosis and reactive hyperplasia of spleen, alveolar atrophy and hemorrhage and other pathological changes. Compared with the Oregon C₂₄V group, the lesion of Z6 group were the most serious and the lesion of DJ2 group was lighter. Using BALB/c mice as experimental animals and the way of intraperitoneal injection, this study can provide data support and guiding significance for the pathogenicity of BVDV-infected mice and the construction of models.

中图分类号:

S852.659.6

阮文强, 陈新诺, 任玉鹏, 覃思楠, 汤承, 张斌. 不同来源的3株牛病毒性腹泻病毒对小鼠的致病性分析[J]. 畜牧兽医学报, 2018, 49(10): 2232-2239.

RUAN Wen-qiang, CHEN Xin-nuo, REN Yu-peng, QIN Si-nan, TANG Cheng, ZHANG Bin. Pathogenicity Analysis of Three Bovine Viral Diarrhea Viruses from Different Sources in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2232-2239.

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不同来源的3株牛病毒性腹泻病毒对小鼠的致病性分析

Pathogenicity Analysis of Three Bovine Viral Diarrhea Viruses from Different Sources in Mice

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