2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

doi:10.11843/j.issn.0366-6964.2018.08.025

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (8): 1789-1796.doi: 10.11843/j.issn.0366-6964.2018.08.025

2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

蒋大秀¹, 马静¹, 王晓泉^1,2, 胡顺林^1,2, 刘晓文^1,2*, 刘秀梵^1,2

1. 扬州大学兽医学院农业部畜禽传染病重点开放实验室, 扬州 225009;
2. 江苏省重点动物传染病和人畜共患病预防控制联合创新中心, 扬州 225009

收稿日期:2018-03-05 出版日期:2018-08-23 发布日期:2018-08-23
通讯作者: 刘晓文,硕士生导师,副教授,E-mail:phontom226@qq.com
作者简介:蒋大秀(1992-),男,山东菏泽人,硕士,主要从事H9N2亚型禽流感流行病学调查及致病机制研究,E-mail:349456530@qq.com
基金资助:
“十二五”农村领域国家科技计划（2015BAD12B01-3）

Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017

JIANG Da-xiu¹, MA Jing¹, WANG Xiao-quan^1,2, HU Shun-lin^1,2, LIU Xiao-wen^1,2*, LIU Xiu-fan^1,2

1. Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Key Joint Center of Infectious Diseases and Zoonosis Prevention and Control of Jiangsu Province, Yangzhou 225009, China

Received:2018-03-05 Online:2018-08-23 Published:2018-08-23

摘要/Abstract

摘要：

活禽市场的家禽，特别是水禽在禽流感病毒（avian influenza virus，AIV）的演化、维持和扩散过程中起重要作用，因此对活禽市场进行长期不间断的流行病学监测非常重要。2016年10月至2017年9月，每月定期采集华东地区某活禽市场家禽喉头和泄殖腔棉拭子，并接种10日龄SPF鸡胚，测定鸡胚尿囊液HA效价，取阳性尿囊液进行HI试验分型，应用RT-PCR对该样品进行全基因PCR并测序，利用DNASTAR、MEGA6.0分析序列，并绘制系统发生树。结果共采集样品1 178份，来自127群，H9N2 AIV阳性样品100份。从水禽中分离到H9N2 AIV 7株，对7株病毒全基因组进行测序和遗传进化分析，不同基因片段均存在不同的遗传进化趋势。活禽市场水禽体内携带的7株H9N2 AIV与当前家禽优势流行基因型一致，因此对活禽市场家禽中循环存在的AIV进行的定期监测，对了解潜在的流行株的演化和出现有重要作用。

Abstract:

Domestic poultry in live poultry markets (LPMs), especially waterfowl, play an important role in the evolution, maintenance and spread of the avian influenza viruses (AIVs). Therefore, it is important to conduct a long-term, regular epidemiological surveillance in the LPMs. From October 2016 to September 2017, we collected oropharyngeal and cloacal swabs from the poultry traded in the LPMs in the eastern China monthly. The samples were inoculated into SPF embryonated chicken eggs. Viruses in the allantoic fluid were confirmed by HA test and were divided to different subtypes by HI test with subtype-specific antibodies. Then eight segments of AIVs' genome were amplified by RT-PCR and these segments were sequenced. Sequence alignment and phylogenetic analysis were performed by software such as DNASTAR and MEGA6.0. A total of 1 178 samples from 127 flocks were collected, and 100 H9N2 AIV strains were isolated, among which 7 strains came from waterfowl. Genetic analysis of eight segments showed that all 7 strains belonged to the S genotype H9N2 AIVs. The genotype of H9N2 viruses from waterfowl traded in LPMs was consistent with that of predominant strains which were circulating in chickens in China. Our study highlights the need to constantly monitor the H9N2 viruses in poultry in LPMs to understand the emergence and evolution of strains that may become epidemic.

中图分类号:

S852.659.5

蒋大秀, 马静, 王晓泉, 胡顺林, 刘晓文, 刘秀梵. 2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析[J]. 畜牧兽医学报, 2018, 49(8): 1789-1796.

JIANG Da-xiu, MA Jing, WANG Xiao-quan, HU Shun-lin, LIU Xiao-wen, LIU Xiu-fan. Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1789-1796.

参考文献

[1] WEBSTER R G, BEAN W J, GORMAN O T, et al. Evolution and ecology of influenza A viruses[J]. Microbiol Rev, 1992, 56(1):152-179.
[2] 谷长勤,罗玲,胡薛英,等. H9N2亚型禽流感病毒诱导蛋鸡免疫器官细胞凋亡的动态变化[J]. 中国兽医学报, 2008, 28(1):12-14.
GU C Q, LUO L, HU X Y, et al. Apoptosis of the immune organ in layers inoculated experimentally with H9N2 subtype avian influenza virus[J]. Chinese Journal of Veterinary Science, 2008, 28(1):12-14. (in Chinese)
[3] 张烨,李晓丹,邹淑梅,等. 2009~2013年我国活禽市场环境样本中禽流感病毒的检测[J]. 病毒学报, 2015, 31(6):615-619.
ZHANG Y, LI X D, ZOU S M, et al. Detection of avian influenza virus in environmental samples collected from live poultry markets in China during 2009-2013[J]. Chinese Journal of Virology, 2015, 31(6):615-619. (in Chinese)
[4] 段晓冬,沈丹,张海明,等. 2013年广州市活禽农贸市场禽流感流行病学调查[J]. 中国动物检疫, 2014, 31(11):33-36, 43.
DUAN X D, SHEN D, ZHANG H M, et al. Epidemiological investigation of avian influenza in retail live poultry markets in Guangzhou in 2013[J]. China Animal Health Inspection, 2014, 31(11):33-36, 43. (in Chinese)
[5] 顾敏,彭大新,刘秀梵. 我国H9N2亚型禽流感病毒的流行和进化特点[J]. 生命科学, 2015, 27(5):531-538.
GU M, PENG D X, LIU X F. Features of the evolution and epidemiology of H9N2 subtype avian influenza virus in China[J]. Chinese Bulletin of Life Sciences, 2015, 27(5):531-538. (in Chinese)
[6] KAGEYAMA T, FUJISAKI S, TAKASHITA E, et al. Genetic analysis of novel avian A(H7N9) influenza viruses isolated from patients in China, February to April 2013[J]. Euro Surveill, 2013, 18(15):20453.
[7] LAM TT Y, WANG J, SHEN Y Y, et al. The genesis and source of the H7N9 influenza viruses causing human infections in China[J]. Nature, 2013, 502(7470):241-244.
[8] LEE R T C, GUNALAN V, VAN T D, et al. A new piece in the puzzle of the novel avian-origin influenza A (H7N9) virus[J]. Biol Direct, 2013, 8:26.
[9] ZHAO G, GU X B, LU X L, et al. Novel reassortant highly pathogenic H5N2 avian influenza viruses in poultry in China[J]. PLoS One, 2012, 7(9):e46183.
[10] GU M, HUANG J Q, CHEN Y X, et al. Genome sequence of a natural reassortant H5N2 avian influenza virus from domestic mallard ducks in eastern China[J]. J Virol, 2012, 86(22):12463-12464.
[11] MI Z Q, LIU W, FAN H, et al. Complete genome sequence of avian influenza virus A/chicken/Jiangsu/1001/2013(H5N2), demonstrating continuous reassortance of H5N2 in China[J]. Genome Announc, 2013, 1(4):e00469-13.
[12] CHEN H Y, YUAN H, GAO R B, et al. Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection:A descriptive study[J]. Lancet, 2014, 383(9918):714-721.
[13] JIN Y, YU D, REN H G, et al. Phylogeography of Avian influenza A H9N2 in China[J]. BMC Genomics, 2014, 15:1110.
[14] 古小兵. 华东地区2011-2012禽流感流行病学调查及近三年H9N2禽流感病毒遗传进化分析[D]. 扬州:扬州大学, 2013.
GU X B. The epidemiology survey of avian influenza virus in eastern China during 2011 to 2012 and the genetic evolution analysis of H9N2 subtype avian influenza virus in last three years[D]. Yangzhou:Yangzhou University, 2013. (in Chinese)

[1]	郭雪莲, 李永琴, 李瑞乾, 李昊, 靳双媛, 王雪妍, 杜家伟, 许立华. 牛呼吸道合胞体病毒G和F蛋白的生物学功能[J]. 畜牧兽医学报, 2024, 55(4): 1478-1487.
[2]	毛秋艳, 周淑宁, 刘朔, 彭程, 尹馨, 张雅馨, 周婉婷, 李金平, 侯广宇, 蒋文明, 宋厚辉, 刘华雷. H3亚型禽流感病毒荧光定量RT-PCR检测方法的建立与应用[J]. 畜牧兽医学报, 2024, 55(3): 1137-1146.
[3]	毕振威, 王文杰, 刘雅坤, 彭大新. 新的犬ANP32A的克隆及其在流感病毒跨物种感染中的作用[J]. 畜牧兽医学报, 2024, 55(2): 660-669.
[4]	孟令宅, 陈春丽, 于蒙蒙, 王占新, 王素艳, 刘鹏, 何塔娜, 郭茹, 陈运通, 刘长军, 祁小乐, 吴志强, 高玉龙. B亚型禽偏肺病毒对黄羽肉鸡致病性分析及其灭活疫苗免疫效果评价[J]. 畜牧兽医学报, 2023, 54(12): 5154-5161.
[5]	蒋盛强, 胡靖, 陈红英. H1N1亚型流感病毒感染A549细胞的环状RNA表达分析[J]. 畜牧兽医学报, 2023, 54(11): 4724-4734.
[6]	张傲, 谭斌, 刘可欣, 刘佳利, 张淑琴. 一株H1N1亚型猪流感病毒全基因组特征分析[J]. 畜牧兽医学报, 2023, 54(11): 4866-4871.
[7]	何辰香, 高闪电, 田占成, 独军政, 王锦明, 关贵全, 殷宏. 基于G_NS蛋白的牛流行热病毒感染与免疫鉴别诊断ELISA方法的建立[J]. 畜牧兽医学报, 2023, 54(10): 4320-4326.
[8]	常益铭, 汤承, 岳华. 牦牛源牛呼吸道合胞体病毒的分子流行病学调查[J]. 畜牧兽医学报, 2023, 54(5): 2030-2041.
[9]	方源, 侯巧弟, 项超辉, 赵红奕, 齐雪峰. IFITM3对小反刍兽疫病毒在山羊子宫内膜上皮细胞中增殖的调控效应[J]. 畜牧兽医学报, 2023, 54(5): 2200-2207.
[10]	李舜, 李桂珍, 原耀贤, 李康健, 马春全, 李守军, 黄良宗, 马骏. PA-X基因的长度变化对H3N2犬流感病毒复制能力和致病性的影响[J]. 畜牧兽医学报, 2023, 54(1): 272-280.
[11]	崔明仙, 王星博, 黄彦铭, 卞希一, 冯梦珂, 颜焰, 董伟仁, 周继勇. 3株H3N2亚型禽流感病毒的基因组特征与演化分析[J]. 畜牧兽医学报, 2022, 53(11): 4116-4122.
[12]	于蒙蒙, 包媛玲, 王素艳, 辛子琪, 刘鹏, 冯笑艳, 孟令宅, 郭茹, 张艳萍, 刘长军, 祁小乐, 李俊平, 王笑梅, 高玉龙. B亚型禽偏肺病毒的分离鉴定及致病性研究[J]. 畜牧兽医学报, 2022, 53(10): 3540-3549.
[13]	周勇, 李知新, 鲁宏伟, 孙燕, 李甜, 杜凡姝, 蒲娟. 我国H5和H7N9亚型高致病性禽流感的监测及疫情暴发分析[J]. 畜牧兽医学报, 2022, 53(9): 3093-3106.
[14]	潘春容, 邓瑞雪, 胡林杰, 朱学亮, 孙跃峰, 王桂荣, 蒙学莲. 组蛋白去乙酰化酶抑制剂对小反刍兽疫病毒复制的影响[J]. 畜牧兽医学报, 2022, 53(7): 2307-2316.
[15]	陈子轩, 张楠, 胡群, 全柯吉, 秦涛, 陈素娟, 彭大新, 刘秀梵. 我国H9N2亚型禽流感病毒血凝素蛋白145和153位抗原位点变异分析[J]. 畜牧兽医学报, 2022, 53(4): 1165-1172.

2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价