畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 863-870.doi: 10.11843/j.issn.0366-6964.2014.06.002

• 遗传繁育 • 上一篇    下一篇

Myf5基因8个SNPs位点与京海黄鸡生长和繁殖性状的关联分析

唐莹1,2,王金玉1,2*,张跟喜1,2,樊庆灿1,2,陈学森1,2,张涛1,2,魏岳1,2,施会强3   

  1. (1.扬州大学动物科学与技术学院,扬州 225009; 2.江苏省动物遗传繁育与分子设计重点实验室,扬州 225009;3.江苏京海集团,南通 226103)
  • 收稿日期:2013-10-25 出版日期:2014-06-23 发布日期:2014-06-23
  • 通讯作者: 王金玉,E-mail:jywang@yzu.edu.cn
  • 作者简介:唐莹(1989- ),女,江苏盐城人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:852376132@qq.com
  • 基金资助:

    江苏省高校自然科学基金(12KJB230003);国家自然科学基金(31201793);国家肉鸡产业技术体系(nycytx-42-G1-05);江苏高校优势学科建设工程;江苏省动物遗传繁育与分子设计重点实验室

Eight SNPs of the Myf5 Gene and Its Relationship with Growth and Reproductive Traits in Jinghai Yellow Chicken

TANG Ying1,2,WANG Jin-yu1,2*,ZHANG Gen-xi1,2,FAN Qing-can1,2,CHEN Xue-sen1,2, ZHANG Tao1,2,WEI Yue1,2,SHI Hui-qiang3   

  1. (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009, China;2.Key Laboratory for Animal Genetics,Breeding,Reproduction and Molecular Design of Jiangsu Province,Yangzhou 225009, China; 3.Jiangsu Jinghai Poultry Industry Group Co.,Ltd.,Nantong 226103, China)
  • Received:2013-10-25 Online:2014-06-23 Published:2014-06-23

摘要:

旨在探讨Myf5基因与鸡生长繁殖性状的关联性。本研究采用PCR-SSCP方法,检测Myf5基因在京海黄鸡群体中的多态性,并对这些多态位点进行单倍型关联分析。结果表明:在Myf5基因外显子处共检测到8个SNPs位点。在379只京海黄鸡的群体中形成了9种单倍型。最小二乘分析结果显示,在生长性状方面,单倍型组合H1H5对8和12周龄体重影响显著(P<0.05),单倍型组合H2H6则对12和14周龄体重影响显著(P<0.05);在繁殖性状方面,单倍型组合H1H5在开产体重上为优势单倍型组合,显著高于单倍型组合H1H4和H2H4(P<0.05),极显著高于单倍型组合H1H3(P<0.01)。就300 d蛋重均值来说,单倍型组合H1H3为劣势单倍型组合;对300 d的产蛋数数据分析显示,单倍型组合H1H3的300 d产蛋数极显著高于H1H4(P<0.01)。因此,京海黄鸡Myf5基因外显子的多态性对其生长和繁殖性状都有一定影响,为探索鸡育种过程中的标记辅助选择提供了参考资料。

Abstract:

The objective of this study was to analyze the association of SNPs between Myf5 gene and chicken growth and reproductive traits.The SNPs of Myf5 gene in Jinghai Yellow chicken were detected by PCR-SSCP method and then the haplotype analysis was done.As a result,8 SNPs were identified in exons of the Myf5 gene.Nine haplotypes were detected in the group of 379 Jinghai Yellow chicken.For growth traits,the least square analysis showed that H1H5 had significant effects on 8 and 12 week-age-weight (P<0.05),H2H6 had significant effects on 12 and 14 week-age-weight (P<0.05).For reproductive traits,H1H5 had higher body weight in first egg than H1H4,H2H4 and H1H3(P<0.05 or P<0.01),of which,the difference between H1H5 and H1H3 was significant(P<0.01).H1H3 had a poor performance in average egg weight of 300 days.On the other hand,H1H3 had an advantage in egg number of 300 days,and higher than that of H1H4(P<0.01).The results show that SNPs of Myf5 have certain effects on growth and reproductive traits in the Jinghai Yellow chicken,and which can be used in marker-assisted selection to accelerate the chicken breeding progress.

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