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  2008  Vol.39 (Number 12)    Dec. 24, 2008
 
 
 
 
 
 
 
 
 
 
 


1625
YIN Zong-jun, ZHANG Qin, DING Xiang-dong, WANG Chun-kao and ZHOU Jie
  Methodology of Genetic Analysis for Discrete Traits of Livestock
    The objective of this paper is to provide an introduction to generalized linear mixed models(GLMM), and to compare the efficiency of heritability estimate for discrete traits using three methods (LM, TM, GLMM). Binary response traits ar...
    2008 Vol. 39 (12): 1625-1630 [Abstract ] ( 47 ) [HTML 0KB] [PDF 1287KB ]( 103 )


1631
XIAO Chao-ting, , DI Ran, CHU Ming-ing, FU Yan, FANG Li, MA Yue-hui, LI Kui and XU Zuo-peng
  Polymorphism and Sequence Analysis of Partial Fragment of BMP6 Gene in Five Sheep Breeds
    Single nucleotide polymorphisms (SNPs) of exons 5, 6, 7 of bone morphogenetic protein 6 (BMP6) gene were detected in 301 individuals of five sheep breeds (Small-tailed Han, Hu, Dorset, Texel and Corriedale sheep) with three pairs of pri...
    2008 Vol. 39 (12): 1631-1639 [Abstract ] ( 33 ) [HTML 0KB] [PDF 1287KB ]( 77 )


1640
HUANG Dan-li, CHEN Ren-jin, YANG Zhang-ping, MAO Yong-jiang, LI Yun-long, TIAN Dai-jun, CHEN Liang and ZHAO Xiao-ong
  Analysis on Associations of SNPs of Leptin Gene with Growth Traits in Four Sheep Breeds
    PCR-SSCP was applied to detect single nucleotide polymorphism (SNP) of exon 2 and exon 3 of the Leptin gene for 358 sheep, including Poll Dorsets (PD), Suffolks (SF), Texels (TX) and Tan sheep (Tan). The polymorphisms were demonstrated,...
    2008 Vol. 39 (12): 1640-1646 [Abstract ] ( 31 ) [HTML 0KB] [PDF 603KB ]( 60 )


1647
LI Mei-u, LI Lan, MIN Ling-jiang and WANG Jian-min
  Genetic Analysis of IGFBP-3 Gene and Its Association with Economic Traits in Goats
    501 goat individuals in seven populations were detected by PCR-RFLP. The results showed the polymorphisms of XspⅠ existed in every population and the number of the individuals with genotype AG was more than that with genotype AA while ...
    2008 Vol. 39 (12): 1647-1653 [Abstract ] ( 22 ) [HTML 0KB] [PDF 454KB ]( 71 )


1654
SUN Rui-ping, WANG Li-in, ZHU Guang-qin, WANG Jian-gang, SONG Yu-uan, LIANG Zhao-i and CAO Bin-un
  Polymorphism of Exon 10 of Prolactin Receptor Gene and Its Relationship with Milk Performance of Xinong Saanen Dairy Goat
    Prolactin receptor (PRLR) gene was studied as a candidate gene for the milk performance of in Xinong Saanen dairy goat. Four pairs of primers were designed to detect single nucleotide polymorphisms of exon 10 of PRLR gene by PCR-SSCP. T...
    2008 Vol. 39 (12): 1654-1660 [Abstract ] ( 28 ) [HTML 0KB] [PDF 1014KB ]( 44 )


1661
JIA Jin, , MA Yan, SUN Dong-iao, ZHANG Yi and ZHANG Yuan
  Association Analysis between DGAT1 Gene and Milk Production Traits in Chinese Holstein
    With PCR-RFLP technique, the polymorphism in the exon8 of DGAT1 gene were investigated in a population of 1 222 Chinese Holstein cows from 17 bull families. Three kinds of genotypes were found and the genotypic frequencies of KK, KA and...
    2008 Vol. 39 (12): 1661-1664 [Abstract ] ( 25 ) [HTML 0KB] [PDF 582KB ]( 64 )


1665
LI Shan-gang, CHEN Xue-jin, LI Hong-bin, WEI Cai-hong and DU Li-in
  The Affection of Different Activation Ways and Culture Medium to the
Parthenogenesis and Development of Rabbit Oocytes
    The development potentiality of the matured Newzeland rabbit oocytes that were activated by different ways and cultured in different media was compared. The result is that the electric pulse or chemical activation could not activate the...
    2008 Vol. 39 (12): 1665-1670 [Abstract ] ( 25 ) [HTML 0KB] [PDF 1020KB ]( 50 )


1671
WANG Yan-hong, WU De, XU Sheng-u, ZHOU Dong-sheng, ZHOU Ping and TAN Xian-i
  Effects of Dietary Energy Level and Source on Oocyte Quality
and Gene Expression in Gilts
    Fifty-four Landrace×Yorkshire crossbred gilts, with initial body weight of （59±4.2）kg, were allotted to 3×2 factorial experiment to study the effects of dietary energy level and source on follicle development, oocyte maturation in ...
    2008 Vol. 39 (12): 1671-1678 [Abstract ] ( 34 ) [HTML 0KB] [PDF 391KB ]( 51 )


1679
SHI Ming-an, , GAO Xue, YANG Xue-i and DOU Zhong-ing
  Isolation, Culture and Identification of Goat Hair Follicle Stem Cells
    Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0.2 mg/mL EDTA. The resultant cells were cultured in self-prep...
    2008 Vol. 39 (12): 1679-1683 [Abstract ] ( 29 ) [HTML 0KB] [PDF 774KB ]( 56 )
 
 


1684
ZHANG Tie-ing, ZHANG Yan-ling, , YAN Su-mei, YIN Yu-long, FAN Ming-he and WANG Jing
  Study on the Determination of Endogenous Outputs and True Digestibility of
Calcium and Phosphorus with Soybean Meal for Growing Pigs by
Linear Regression Analysis Technique
    This study was designed to determine endogenous fecal calcium and phosphorus losses and true fecal calcium and phosphorus digestibility with soybean meal for growing barrows by the Linear Regression Analysis Technique (REG). Six Yorkshi...
    2008 Vol. 39 (12): 1684-1691 [Abstract ] ( 29 ) [HTML 0KB] [PDF 401KB ]( 51 )


1692
GE Chang-rong, ZHAO Su-mei, ZHANG Xi, LAI Hua, LI Chang-qiang and GAO Shi-heng
  Effect of Dietary Protein Levels on Meat Quality in Wujin Pig
    The aim of the study was to investigate the effect of dietary protein levels on meat quality so as to find the suitable dietary protein level according to the best meat quality by Fuzzy Comprehensive Judgment Model. Ninety pigs were ran...
    2008 Vol. 39 (12): 1692-1700 [Abstract ] ( 23 ) [HTML 0KB] [PDF 426KB ]( 49 )


1701
YANG Fei-un, , CHEN Dai-en, HUANG Jin-iu and LIU Zuo-hua
  Developmental Changes of Myofiber Types in Longissimus Dorsi Muscle of
Rongchang and DLY Pigs under Different Nutrient Condition
    This experiment was conducted to study the developmental changes of myofiber types in longissimus dorsi (LD) muscle of Rongchang (RC) and Duroc×Landrace×Yorkshire (DLY) pigs under different dietary nutrient condition, by determining t...
    2008 Vol. 39 (12): 1701-1708 [Abstract ] ( 32 ) [HTML 0KB] [PDF 499KB ]( 48 )


1709
XU Qing-fang, , YU Zhu, LI Sheng-li, HAN Jian-guo, BAI Chun-sheng, XUE Yan-lin, BAI Jing-long and HUANG Yi
  The Effect of Formic Acid or Previous Fermented Juice on Baled Afalfa Silage
    The effects of formic acid and previous fermented juice on fermentation and nutrient of baled alfalfa silage were studied. The wilted forage as CK, the amount of formic acid and previous fermented juice were 6 L/t and 2 L/t, respectivel...
    2008 Vol. 39 (12): 1709-1714 [Abstract ] ( 31 ) [HTML 0KB] [PDF 374KB ]( 42 )
 
 


1715
YANG Chun-iao, WANG Xiao-e, LIU Guan-hua, XIAO Hai-jun, CUI Jin-sheng and ZHU Rui-liang
  Establishment and Application of Immunofluorescence Assay and
Indirect ELISA for Detection of B.avium
    In this study, out membrane proteins of Bordetella avium strains P5 and P8 were prepared by improved Wooldridge method. The contents of Bordetella avium OMP were determined by Bradford method. SDS-PAGE electrophoresis was performed to i...
    2008 Vol. 39 (12): 1715-1720 [Abstract ] ( 24 ) [HTML 0KB] [PDF 648KB ]( 51 )


1721
SHEN Yan, HUA Xiu-guo, CUI Li, ZHU Jian-guo and YANG Zhi-biao
  Effect of Experimental Infection with Two SS2 Strains on
Several Immune Indices in Miniature Swine
    To investigate the effect of infection of different Streptococcus suis strains (HA9801, ZY05719) on the blood biochemical indices and immunological state in Bama miniature pigs，bacterial content and leucocyte of blood were determined a...
    2008 Vol. 39 (12): 1721-1730 [Abstract ] ( 23 ) [HTML 0KB] [PDF 1731KB ]( 60 )


1731
HAN Xiu-e, , ZHANG Ping, WANG Xue-feng, KONG Xian-gang, XIANG Wen-hua and ZHOU Jian-hua
  Construction of an Infectious Clone within the V3 Region of gp90 Surface
Protein of EIAV by N-glycosylation Reversmutations
    The Chinese equine infectious anemia virus (EIAV) donkey-leukocyte attenuated vaccine (DLV) provides a unique natural model system, by which the mechanism of virulent attenuation and the immunological control of lentivirus replic...
    2008 Vol. 39 (12): 1731-1736 [Abstract ] ( 26 ) [HTML 0KB] [PDF 784KB ]( 40 )


1737
XU Xiang-ming, ZHU Shan-uan, ZHANG Quan, XUE Zheng-feng and LI Hou-da
  The Analysis of the Immune Enhancing Effects of CpG-ODN on the Recombinant Plasmid which Contain the H Protein of Canine Distemper Virus
    To enhance the immunocompetence of recombinant plasmid against canine distemper virus, four CpG-oligodeoxynueleotides (CpG-ODN) were designed and used in this research. Following lymphocytes proliferation test determined by 3H-TdR incor...
    2008 Vol. 39 (12): 1737-1742 [Abstract ] ( 28 ) [HTML 0KB] [PDF 516KB ]( 48 )


1743
DONG Li-na, GAO Feng-shan, XU Chong-bo, HU Gui-ue and JIANG Hai-long
  Construction and Identification of the Expression Recombinants for
PEDV COE Gene in Lactococcus Lactis
    The partial S glycoprotein gene of porcine epidemic diarrhea virus (PEDV) was amplified by RT-PCR and sequenced. The gene consisted of 531 bp and shared 99.4% nucleotide homology with PEDV CV777 strain (AF353511) published on GenBank. T...
    2008 Vol. 39 (12): 1743-1747 [Abstract ] ( 20 ) [HTML 0KB] [PDF 767KB ]( 45 )


1748
PENG Xiu-li, , GAO Jun-ei, HU Si-shun, HU Fu-li and BI Ding-ren
  Binding Research Between the Tissues Membrane of Chick Embryo and pMGA
    The GST-pMGA was expressed in E. coli BL21 with the pGEX fusion expression system. GST-pMGA expressed in supernatant was purified using GST Bind Resin and a plenty of soluble protein was obtained, the final preparation was 96% after pur...
    2008 Vol. 39 (12): 1748-1752 [Abstract ] ( 27 ) [HTML 0KB] [PDF 827KB ]( 46 )
 
 


1753
GONG Yong-qiang, CHENG An-chun, , WANG Ming-shu, , YANG Mei, ZHANG Yao, CHEN Bin, and ZHONG Xiao-rong
  Preliminary Studies on Prokaryotic Expression,Refolding of Duck IFN-α
Mature Peptide and Its Activity against Virus
    In order to obtain DuIFN-α and investigate its biological function, duck interferon-α(DuIFN-α) mature protein gene was cloned in to pMD18-T vector and inserted into the expressing plasmid/ pET32a+. Then,this recombinant pl...
    2008 Vol. 39 (12): 1753-1758 [Abstract ] ( 22 ) [HTML 0KB] [PDF 541KB ]( 50 )


1759
LIU Wen-jie, CHEN Yao-ing, WANG Zi-u, DONG Yu-lan, CAO Jing, XIE Dian and JIA Liu-jun
  Effect of Monochromatic Light on the Muscle Growth and Muscle
Fiber Development and Testosterone Secretion in Broilers
    The effect of monochromatic light on muscle growth and muscle fiber development in broilers were studied, and the internal mechanism was discussed in this paper. A total of 260 newly hatched broilers were divided randomly into four ligh...
    2008 Vol. 39 (12): 1759-1764 [Abstract ] ( 29 ) [HTML 0KB] [PDF 1142KB ]( 51 )


1765
HE Xiao-an, ZHU Zhi-ei, YOU Rong-li, HAO Huan-qing, FAN Rui-en, GAO Li, CAO Jing, DONG Yan-jun and DONG Chang-sheng
  The Study on the Distribution of PRL and Its Receptor in Skin of
Young Male Alpaca(Lama Pacos)
    Alpaca is one of the most important hair-producing animals. However, the genetic regulation of the growth of alpaca hair is largely unknown. Here, we report a surprising finding that prolactin (PRL), PRL receptor (PRL-R) and other PRL r...
    2008 Vol. 39 (12): 1765-1771 [Abstract ] ( 26 ) [HTML 0KB] [PDF 1219KB ]( 55 )


1772
WANG Ji-cang, , MA Su-ing, , WANG Da-ju, HAN He-ou and LIU Deng-cai
  Simultaneous Determination of Nitroimidazole Residues in Swine Meat by Gas
Chromatography-Ion Trap Tandem Mass Spectrometry
    A gas chromatography-ion trap tandem mass spectrometry method was developed for the determination of DMZ, RNZ, SNZ, MNZ and ONZ residues in swine muscle. The nitroimidazole residues were extracted with dichloromethane, followed by reext...
    2008 Vol. 39 (12): 1772-1778 [Abstract ] ( 30 ) [HTML 0KB] [PDF 406KB ]( 42 )
 
 


1779
GENG Rong-qing, , CHANG Hong, LI Yong-hong, JI De-jun, CHANG Chun-fang, WANG Lan-ping and CHANG Guo-bin
  Variation of Complete Coding Sequence of GH Gene in Chinese Bovinae Species
    CDS of GH gene of 5 bovine species Bos taurus, Bos indicus, Bos grunniens, Bos frontalis and Bubalus bubalis was connected from PCR fragments by standard double-strand DNA sequencing method to analyze the variation and molecular evoluti...
    2008 Vol. 39 (12): 1779-1784 [Abstract ] ( 35 ) [HTML 0KB] [PDF 381KB ]( 55 )


1785
LI Yong-hu and KATSUNORI Sato
  Improved and Selective Breeding in Low Nitrogen Excretion Quails
    An experiment was conducted to breed low-excretion type Japanese quails for decreasing the environmental pollution source. Japanese quails from Normal ，Brown breeds and crossing group were used in this eight generations (6-20 week-old)...
    2008 Vol. 39 (12): 1785-1793 [Abstract ] ( 30 ) [HTML 0KB] [PDF 579KB ]( 47 )

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