新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定

doi:10.11843/j.issn.0366-6964.2019.04.022

Abstract

Abstract:

The objective of this study was to investigate the phenotypic and genotypic basis of bovine Shiga toxin-producing Escherichia coli (STEC) from Xinjiang and obtain the evolution and spread of STECs resistance. Susceptibility testing to 18 antimicrobials was performed on bovine (non-O157:H7)STEC isolates from 6 regions of Xinjiang. Meanwhile, the extended spectrum β-lactamases(ESBL) genes were amplified. In this study 4.31% STEC isolates were multidrug resistant to antimicrobials (MDR), and 1.91% were ESBLs-producing strains. The predominant ESBL genes detected were bla_TEM and bla_CTX-M. This is the first report of bla_TEM and bla_CTX-M in STEC isolates in Xinjiang. Most resistant STECs (91.67%) isolated in this study belong to phylogenetic groups A. These findings suggest that MDR STECs are emerging as a result of nonpathogenic E. coli acquiring virulence and resistance genes. This may convey a certain competitive advantage for the colonization of these STECs in cattle when antimicrobial selective pressures are present, potentially leading to an increase in contamination of food with resistant STECs.

Key words: Shiga toxin-producing Escherichia coli, drug resistance, foodborne pathogen, antimicrobial, ESBLs

CLC Number:

TONG Panpan, MA Kaiqi, LIU Zhenghui, XIE Jinxin, SU Hong, WANG Dong, SUN Xue, GAO Jiaojiao, SU Zhanqiang. Characterization of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Genes in Bovine Origin Shiga Toxin-producing Escherichia coli in Xinjiang[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 887-892.

References

[1] MUGHINI-GRAS L, VAN PELT W, VAN DER VOORT M, et al. Attribution of human infections with Shiga toxin-producing Escherichia coli (STEC) to livestock sources and identification of source-specific risk factors, the Netherlands (2010-2014)[J]. Zoonoses Public Health, 2018, 65(1):e8-e22.
[2] ABDISSA R, HAILE W, FITE A T, et al. Prevalence of Escherichia coli O157:H7 in beef cattle at slaughter and beef carcasses at retail shops in Ethiopia[J]. BMC Infect Dis, 2017, 17:277.
[3] KARMAIL M A, MASCARENHAS M, SHEN S H, et al. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease[J]. J Clin Microbiol, 2003, 41(11):4930-4940.
[4] GOULD L H, MODY R K, ONG K L, et al. Increased recognition of non-O157 Shiga toxin-producing Escherichia coli infections in the United States during 2000-2010:epidemiologic features and comparison with E. coli O157 infections[J]. Foodborne Pathog Dis, 2013, 10(5):453-460.
[5] BEUTIN L, FACH P. Detection of Shiga toxin-producing Escherichia coli from nonhuman sources and strain typing[J/OL]. Microbiol Spectr, 2014, 2(3):doi:10.1128/microbiolspec.EHEC-0001-2013[2019-02-06]. http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.EHEC-0001-2013.
[6] AMÉZQUITA-LÍPEZ B A, SOTO-BELTRÁN M, LEE B G, et al. Isolation, genotyping and antimicrobial resistance of Shiga toxin-producing Escherichia coli[J]. J Microbiol Immunol Infect, 2018, 51(4):425-434.
[7] KARIYAWASAM S, JOHNSON T J, NOLAN L K. Unique DNA sequences of avian pathogenic Escherichia coli isolates as determined by genomic suppression subtractive hybridization[J]. FEMS Microbiol Lett, 2006, 262(2):193-200.
[8] D'ANDREA M M, ARENA F, PALLECCHI L, et al. CTX-M-type β-lactamases:a successful story of antibiotic resistance[J]. Int J Med Microbiol, 2013, 303(6-7):305-317.
[9] BUSH K, JACOBY G A, MEDEIROS A A. A functional classification scheme for β-lactamases and its correlation with molecular structure[J]. Antimicrob Agents Chemother, 1995, 39(6):1211-1233.
[10] THOMSON K S. Extended-spectrum-β-lactamase, AmpC, and Carbapenemase issues[J]. J Clin Microbiol, 2010, 48(4):1019-1025.
[11] JOUINI A, VINUÉ L, SLAMA K B, et al. Characterization of CTX-M and SHV extended-spectrum β-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia[J]. J Antimicrob Chemother, 2007, 60(5):1137-1141.
[12] DIWAN V, CHANDRAN S P, TAMHANKAR A J, et al. Identification of extended-spectrum β-lactamase and quinolone resistance genes in Escherichia coli isolated from hospital wastewater from central India[J]. J Antimicrob Chemother, 2012, 67(4):857-859.
[13] SMET A, MARTEL A, PERSOONS D, et al. Broad-spectrum β-lactamases among Enterobacteriaceae of animal origin:molecular aspects, mobility and impact on public health[J]. FEMS Microbiol Rev, 2010, 34(3):295-316.
[14] CLERMONT O, CHRISTENSON J K, DENAMUR E, et al. The Clermont Escherichia coli phylo-typing method revisited:improvement of specificity and detection of new phylo-groups[J]. Environ Microbiol Rep, 2013, 5(1):58-65.
[15] 赵志晶, 刘秀梅. 食品样品中大肠杆菌O157:H7复合PCR检测方法的研究[J]. 卫生研究, 2004, 33(6):716-719.
ZHAO Z J, LIU X M. Multiplex PCR assay against E. coli O157 in foodstuffs[J]. Journal of Hygiene Research, 2004, 33(6):716-719. (in Chinese)
[16] 叶青,张雪寒,何孔旺,等. 牛源大肠杆菌O157:H7的分离及毒力基因鉴定[J]. 中国兽医学报, 2012, 32(8):1148-1153.
YE Q, ZHANG X H, HE K W, et al. Isolation of Escherichia coli O157:H7 from cattle and detection of its virulence genes[J]. Chinese Journal of Veterinary Science, 2012, 32(8):1148-1153. (in Chinese)
[17] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; Twenty-third informational supplement[R]. PA:CLSI, 2013.
[18] TONG P P, SUN Y, JI X, et al. Characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in Escherichia coli isolated from chickens[J]. Foodborne Pathog Dis, 2015, 12(4):345-352.
[19] BREHONY C, CULLINAN J, CORMICAN M, et al. Shiga toxigenic Escherichia coli incidence is related to small area variation in cattle density in a region in Ireland[J]. Sci Total Environ, 2018, 637-638:865-870.
[20] KINTZ E, BRAINARD J, HOOPER L, et al. Transmission pathways for sporadic Shiga-toxin producing E. coli infections:a systematic review and meta-analysis[J]. Int J Hyg Environ Health, 2016, 220(1):57-67.
[21] NAKAMURA H, IGUCHI A, MAEHARA T, et al. Comparison of three molecular subtyping methods among O157 and non-O157 Shiga toxin-producing Escherichia coli isolates from Japanese cattle[J]. Jpn J Infect Dis, 2017, 71(1):45-50.
[22] PAQUETTE S J, STANFORD K, THOMAS J, et al. Quantitative surveillance of Shiga toxins 1 and 2, Escherichia coli O178 and O157 in feces of western-Canadian slaughter cattle enumerated by droplet digital PCR with a focus on seasonality and slaughterhouse location[J]. PLoS One, 2018, 13(4):e0195880.
[23] CLERMONT O, BONACORSI S, BINGEN E. Rapid and simple determination of the Escherichia coli phylogenetic group[J]. Appl Environ Microbiol, 2000, 66(10):4555-4558.
[24] ALIZADE H, GHANBARPOUR R, NEKOUBIN M. Phylogenetic of Shiga toxin-producing Escherichia coli and a typical enteropathogenic Escherichia coli strains isolated from human and cattle in Kerman, Iran[J]. Int J Entric Pathog, 2014, 2(1):e15195.
[25] KENNEDY C A, FANNING S, KARCZMARCZYK M, et al. Characterizing the multidrug resistance of non-O157 Shiga toxin-producing Escherichia coli isolates from cattle farms and abattoirs[J]. Microb Drug Resist, 2017, 23(6):781-790.
[26] YUAN L, LIU J H, HU G Z, et al. Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China[J]. J Med Microbiol, 2009, 58:1449-1453.
[27] LANDERSDORFER C B, NATION R L. Colistin:how should it be dosed for the critically ill?[J]. Semin Respir Crit Care Med, 2015, 36(1):126-135.
[28] LIU Y Y, WANG Y, WALSH T R, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China:a microbiological and molecular biological study[J]. Lancet Infect Dis, 2016, 16(2):161-168.
[29] XAVIER B B, LAMMENS C, RUHAL R, et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016[J/OL]. Euro Surveill, 2016, 21(27):doi:10.2807/1560-7917.ES.2016.21.27.30280[2019-02-06]. https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2016.21.27.30280.
[30] SMILLIE C, GARCILLÁN-BARCIA P, FRANCIA M V, et al. Mobility of plasmids[J]. Microbiol Mol Biol Rev, 2010, 74(3):434-452.

Characterization of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Genes in Bovine Origin Shiga Toxin-producing Escherichia coli in Xinjiang

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Xueliang, WANG Shuyi, SUN Ke, SU Qian, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis of Albendazole-susceptible and Resistant Strains of Haemonchus contortus by RNA-Seq [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1940-1944.
[2]	TONG Panpan, MA Kaiqi, LIU Zhenghui, XIE Jinxin, SU Hong, WANG Dong, SUN Xue, GAO Jiaojiao, SU Zhanqiang. Characterization of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Genes in Bovine Origin Shiga Toxin-producing Escherichia coli in Xinjiang [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 887-892.
[3]	ZHAO Xueliang, WANG Shuyi, HU Hebateer, SUN Ke, SU Qian, Lü Xu, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 637-644.
[4]	ZHAO Xueliang, WANG Shuyi, HU Hebateer, SUN Ke, SU Qian, Lü Xu, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 637-644.
[5]	SHANG Lijun, YANG Tianren, YU Haitao, HUANG Shuo, ZENG Xiangfang, QIAO Shiyan. Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 406-414.
[6]	SHANG Lijun, YANG Tianren, YU Haitao, HUANG Shuo, ZENG Xiangfang, QIAO Shiyan. Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 406-414.
[7]	ZHANG Hang, YANG Feng, LI Xinpu, WANG Xurong, LUO Jinyin, LI Hongsheng. Study on Relationship between Antimicrobial Resistance and Efflux Pump Related Genes of Methicillin-Resistant Staphylococcus aureus in Dairy Cow [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2326-2332.
[8]	YANG Aining, MA Xiaoping, GU Yu, YU Yan, HU Jing, JIANG Yaozhang, PENG Guangneng, ZHONG Zhijun, ZUO Zhicai. The Interaction between Farnesol (Quorum Sensing Molecule) and Candida albicans [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 28-36.
[9]	WU Yiran, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 151-158.
[10]	YANG Aining, MA Xiaoping, GU Yu, YU Yan, HU Jing, JIANG Yaozhang, PENG Guangneng, ZHONG Zhijun, ZUO Zhicai. The Interaction between Farnesol (Quorum Sensing Molecule) and Candida albicans [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 28-36.
[11]	WU Yiran, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 151-158.
[12]	CAI Jun-jun, YUAN Tian-qing, YANG Yin-ping, GAO Shuang, GONG Xing-wen. Virtual Screening and Activity Evaluation of Multi-target Antibacterial Lead Compounds [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1500-1510.
[13]	CAI Jun-jun, YUAN Tian-qing, YANG Yin-ping, GAO Shuang, GONG Xing-wen. Virtual Screening and Activity Evaluation of Multi-target Antibacterial Lead Compounds [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1500-1510.
[14]	YU Yan-chao, WANG Li-li, PAN Qiao, XIN Jiu-qing, WANG Xiu-mei. Construction and Biological Characteristics of Salmonella Enterica Serovar Enteritidis luxS Mutant [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1291-1298.
[15]	YU Yan-chao, WANG Li-li, PAN Qiao, XIN Jiu-qing, WANG Xiu-mei. Construction and Biological Characteristics of Salmonella Enterica Serovar Enteritidis luxS Mutant [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1291-1298.