敲除小RNA gcvB后沙门菌的转录组分析

doi:10.11843/j.issn.0366-6964.2019.04.017

Abstract

Abstract:

In this article, high-throughput transcriptome sequencing (RNA-seq) technology was used to screen potential regulatory target genes of small RNA (sRNA) gcvB in Salmonella. Comparative analysis of mRNA in wild-type strain and knockout small RNA gcvB strain has been used. The number and type of GcvB target genes were comprehensively predicted, the main functions and the biological processes of the selected genes were compared by the GO and KEGG databases for further analysis. Some differentially expressed genes were verified by real-time PCR. The results showed that there were 1 244 differential expression genes have been screened out, including 678 up-regulated genes and 566 down-regulated genes. GO enrichment analysis showed that the differentially expressed genes mainly involved molecular functions such as redox activity, iron binding, locomotor activity, cellular components of bacterial flagella, cellular respiration, cobalamin metabolism, and cobalamin biosynthesis. KEGG database analysis showed that differential expression genes mainly participated in 14 signaling pathways, including bacterial chemotaxis, porphyrin and chlorophyll metabolism, microbial metabolism in different environments, two-component systems and methane metabolism. Ten differentially expressed genes in WT strain and ΔGcvB strain were selected from transcriptome analysis and carried out the further Real-time PCR test, and the results show that the trend of gene expression is consistent in both transcriptome analysis and in Real-time PCR test. The study lays the foundation for further exploration of the interaction between sRNA GcvB and target genes, the regulation of sRNA and the pathogenic mechanism in Salmonella.

Key words: Salmonella, small RNA gcvB, transcriptome sequencing, target genes

CLC Number:

S852.61

LIU Lijuan, DONG Ranran, WANG Kaigong, CHENG Zhentao, WEN Ming, WEN Guilan, LI Chen, YANG Qi, ZHOU Bijun. mRNA-Seq Whole-transcriptome Analysis of sRNA gcvB Deletion Background in Salmonella[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 840-850.

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mRNA-Seq Whole-transcriptome Analysis of sRNA gcvB Deletion Background in Salmonella

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