酿酒酵母β-葡聚糖通过膜受体Dectin-1和TLR-2诱导绵羊瘤胃上皮细胞表达SBD-1的机制研究

doi:10.11843/j.issn.0366-6964.2019.04.011

Abstract

Abstract:

The aim of this study was to explore the possible role of TLR-2 and Dectin-1 in the expression of β-defensin-1 (SBD-1) in ovine ruminal epithelial cells (ORECs) induced by Saccharomyces cerevisiae β-glucan. The immunohistochemistry, RT-PCR, immunofluorescence and Western blot were used to detect the expression of Dectin-1 in ORECs, and qPCR and Western blot were used to detected the expression changes of Dectin-1 and TLR-2 after β-glucan stimulated ORECs. Then, ORECs were pretreated using Dectin-1 blocker laminarin or TLR-2 blocking antibody with different concentrations, and the expression of SBD-1 were detected by qPCR and ELISA to determine the involvement of Dectin-1 and TLR-2 in the induction expression of SBD-1 induced by β-glucan. The results showed that:1) Dectin-1 was expressed in ovine ruminal tissues and ORECs, and the expression levels of Dectin-1 and TLR-2 were significantly increased after β-glucan stimulating ORECs (P<0.05); 2)The laminarin or TLR-2 blocking antibody with different concentrations significantly decreased the expression of SBD-1 induced by β-glucan (P<0.01), and this effect became stronger as the laminarin and blocking antibodies concentration increased. The results indicate that Dectin-1 is expressed in both ovine ruminal tissues and ORECs, and the Saccharomyces cerevisiae β-glucan inducing SBD-1 expression in ORECs is mediated by Dectin-1 and TLR-2.

Key words: β-glucan, SBD-1, ovine ruminal epithelial cells, Dectin-1, TLR-2

CLC Number:

S826.5

ZHANG Man, JIN Xin, WANG Yunhe, WEI Fang, WEN Jingyi, YANG Yinfeng. Mechanism of Saccharomyces Cerevisiae β-glucan Inducing SBD-1 Expression in Ovine Rumen Epithelial Cells by Membrane Receptors Dectin-1 and TLR-2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 781-790.

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Mechanism of Saccharomyces Cerevisiae β-glucan Inducing SBD-1 Expression in Ovine Rumen Epithelial Cells by Membrane Receptors Dectin-1 and TLR-2

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