日粮添加油脂和出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2019.04.010

Abstract

Abstract:

This study aimed to investigate the effects of dietary soybean oil supplementation and different birth types on collagen characteristics and related genes expression in muscle of lamb. Sixty-four male Hu lambs with twin born (TW, 32 lambs) and triplet born (TR, 32 lambs) types weaned at 2 months old were selected and fed basal diet (CON) or diet with 2% soybean oil supplemented (SBO). Lambs were randomly divided into 4 treatments (TW-CON, TW-SBO, TR-CON, TR-SBO) according to 2×2 factorial experimental design, each treatment had 16 biological replication, and 1 lamb in each replication. Lambs were slaughtered after 90 days of feeding, and longissimus dorsi muscle samples were collected for detecting the content of collagen, enzymes activity and muscle development-related genes expression level. The results indicated that:1) The collagen type Ⅰ content in TW groups was significantly higher than that in TR groups (P<0.05). Dietary soybean oil supplementation increased collagen content (0.05 < P < 0.10), while had no significant effect on collagen solubility (P>0.05). 2) Matrix metalloproteinases-13 (MMP-13) content decreased significantly with diet soybean oil supplementation (P<0.05), while pyridinoline (PYD) and tissue inhibitor of metalloproteinases (TIMP-1) were affected significantly by the interaction effects between dietary soybean oil supplementation and birth types (P<0.05). 3) MYOD1 expression level was significantly higher in TW groups than that in TR groups (P<0.05). The expression level of FN1 decreased significantly with soybean oil supplemented in diet (P<0.05), whereas FGF2 and COL3A1 expression levels were affected by the interaction effects between dietary soybean oil supplementation and birth types (0.05 < P < 0.10). In addition, the expression levels of FGFR1, TGFβ1 and COL1A1 were not significantly affected by the soybean oil supplementation or birth types (P>0.05). In conclusion, twin born lambs contain more collagen in muscle than triplet born lambs, and soybean oil supplementation show a trend to increase the collagen content in muscle. Moreover, dietary soybean oil supplementation and birth types are combined together to regulate the content of collagen metabolism enzymes and related genes expression level, and then modulate the collagen content in muscle.

Key words: dietary soybean oil, birth types, collagen, gene expression, lamb

CLC Number:

S826.5

WANG Bo, DIAO Zhicheng, MA Yong, LI Fadi, QU Yanghua, XU Chenchen, LU Xiaonan, LUO Hailing. Effects of Dietary Oil Supplementation and Birth Types on Muscle Collagen Characteristics and Related Genes Expression in Muscle of Lamb[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 771-780.

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Effects of Dietary Oil Supplementation and Birth Types on Muscle Collagen Characteristics and Related Genes Expression in Muscle of Lamb

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