ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2019, Vol. 50 ›› Issue (4): 727-737.doi: 10.11843/j.issn.0366-6964.2019.04.006

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Endoplasmic Reticulum Stress Marker Gene Grp78 Is Involved in Regulation of Immune/Inflammatory State of Goose Fatty Liver

LIU Tongjun, ZHAO Pan, ZHAO Minmeng, LIU Long, CUI Hengmi, GONG Daoqing, GENG Tuoyu*   

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
  • Received:2018-10-23 Online:2019-04-23 Published:2019-04-23

Abstract:

The aim of this study was to investigate whether endoplasmic reticulum stress (ERS) marker gene Grp78 was involved in regulation on the expression of inflammation/immunity-related genes in goose fatty liver. Thirty healthy 70-day-old Landaise geese (male or female) with similar body weight were randomly divided into the control group (n=15) and the overfeeding group (n=15). The relative expression of Grp78 in different tissues at different overfeeding periods (7, 14, 19 d) was determined by fluorescent quantitative PCR to clarify the relationship between Grp78 and geese fatty liver formation; Grp78 was overexpressed in goose primary hepatocytes, and the signaling pathways and genes affected by Grp78 were analyzed by RNA-seq (n=3); The expression of genes related to inflammation/immunity was determined in goose fatty liver by fluorescent quantitative PCR (n=4) to clarify the association of these genes with Grp78. The results showed that the expression of Grp78 in the liver and abdominal fat was affected by overfeeding (decreased in liver and increased in abdominal fat). The result of overexpression of Grp78 gene suggested that ERS not only participated in the known metabolic diseases and cell apoptosis pathways, but also had an effect on signal transduction and immune pathways. The Toll-like receptor signaling pathway, TNFα signaling pathway and NF-kappa B signaling pathway were the most prominent ones in regard to immunity/inflammation. In addition, the expression of immune/inflammation related genes, Tnfsf10 and Map3k7cl, was affected by Grp78 overexpression in the goose primary hepatocytes and was affected by overfeeding in goose fatty liver. In summary, this study revealed a new biological function of Grp78/ERS, that is, to regulate cellular immune/inflammatory state and participate in the formation of goose fatty liver.

Key words: nonalcoholic fatty liver, endoplasmic reticulum stress, immunity, goose, Tnfsf10, Map3k7cl

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