转录组揭示禁水环境下双峰驼结肠组织适应机制

doi:10.11843/j.issn.0366-6964.2018.11.007

Abstract

Abstract:

The aim of this study was to understand the mechanism of tissue environmental adaptation in the absence of water in Camelus. In this study, six 6-9 years old adult Alashan Bactrian camels were randomly divided into 2 groups, the first group was the control group (colon1_3), in which the animals were fed with normal feed and water, the second group was the no drinking water group (colon3), in which the animals were in the absence of water for 24 d, other treatment (forage) was the same as the control group. The Illumina HiSeq 2000 sequencing platform was used to sequence the transcriptome of Bactrian camel colon tissue in the control and no drinking water groups, and the transcriptome data were used to perform quality-controlled, alignment, differentially expressed, GO and KEGG analysis. The results showed that, compared with control group, a total of 2 122 differentially expressed genes were obtained in the no drinking water group, among which 813 genes were up-regulated and 1 309 genes were down-regulated. GO enrichment analysis results showed that 30 terms in the no drinking water group were significantly enriched by down-regulated expression genes. There was no statistically significant enrichment term by the up-regulated expression genes. The KEGG pathway analysis showed that the down-regulated expression genes in no drinking water group were significantly enriched in multiply pathways, including spliceosome, protein export, protein processing in endoplasmic reticulum, RNA transport, ribosome biogenesis in eukaryotes, mRNA detection, RNA degradation metabolic pathways. The up-regulated expression genes in no drinking water group were enriched in focal adhesion, lysosome function, and so on. The results of this study indicate that the number of down-regulated expression genes in colon are more than up-regulated expressed genes in water-deficient environments, and the functions of these down-regulated expression genes are mainly associated with RNA processes and protein synthesis pathways. These genes are favorable for camels to reduce the RNA synthesis and the metabolic rate of colon tissue in water-deficient environments.

Key words: bactrian camel, colon, transcriptome, water metabolism

CLC Number:

S824.2

LING Yu, QI Yu, CAO Jun-wei, WANG Shen-yuan, ZHOU Huan-min, ZHANG Yan-ru. Transcriptome Reveals the Adapting Mechanism of Colon Tissues of Bactrian Camel in Water-deficient Environment[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2359-2370.

References

[1] SEQUENCING T B C G, CONSORTIUM A. Genome sequences of wild and domestic bactrian camels[J]. Nat Commun, 2012, 3(1):1202.
[2] WU H G,GUANG X M,AL-FAGEEH M B,et al.Camelid genomes reveal evolution and adaptation to desert environments[J].Nat Commun,2014,5:5188.
[3] MILLER T L,WOLIN M J.Pathways of acetate,propionate,and butyrate formation by the human fecal microbial flora[J].Appl Environ Microbiol,1996,62(5):1589-1592.
[4] MCNEIL N I.The contribution of the large intestine to energy supplies in man[J].Am J Clin Nutr,1984,39(2):338-342.
[5] WAHL M C,WILL C L,LÜHRMANN R.The spliceosome:design principles of a dynamic RNP machine[J].Cell,2009,136(4):701-718.
[6] RITCHIE D B,SCHELLENBERG M J,MACMILLAN A M.Spliceosome structure:piece by piece[J].Biochim Biophys Acta,2009,1789(9-10):624-633.
[7] NG S Y M,CHABAN B,VANDYKE D J,et al.Archaeal signal peptidases[J].Microbiology,2007,153(Pt2):305-314.
[8] DEJGAARD K,THEBERGE J F,HEATH-ENGEL H,et al.Organization of the Sec61 translocon,studied by high resolution native electrophoresis[J].J Proteome Res,2010,9(4):1763-1771.
[9] MÄÄTTÄNEN P,GEHRING K,BERGERON J J M,et al.Protein quality control in the ER:the recognition of misfolded proteins[J].Semin Cell Dev Biol,2010,21(5):500-511.
[10] STOLZ A,WOLF D H.Endoplasmic reticulum associated protein degradation:a chaperone assisted journey to hell[J].Biochim Biophys Acta,2010,1803(6):694-705.
[11] SUZUKI T,IZUMI H,OHNO M.Cajal body surveillance of U snRNA export complex assembly[J].J Cell Biol,2010,190(4):603-612.
[12] PALANCADE B,DOYE V.Sumoylating and desumoylating enzymes at nuclear pores:underpinning their unexpected duties?[J].Trends Cell Biol,2008,18(4):174-183.
[13] MANDEL C R, BAI Y, TONG L. Protein factors in pre-mRNA 3'-end processing[J]. Cell Mol Life Sci, 2008, 65(7-8):1099-1122.
[14] MAQUAT L E. Nonsense-mediated mRNA decay:splicing, translation and mRNP dynamics[J]. Nat Rev Mol Cell Biol, 2004, 5(2):89-99.
[15] AMRANI N, SACHS M S, JACOBSON A. Early nonsense:mRNA decay solves a translational problem[J]. Nat Rev Mol Cell Biol, 2006, 7(6):415-425.
[16] EULALIO A, BEHM-ANSMANT I, IZAURRALDE E. P bodies:at the crossroads of post-transcriptional pathways[J]. Nat Rev Mol Cell Biol, 2007, 8(1):9-22.
[17] CLEMENT S L, LYKKE-ANDERSEN J. No mercy for messages that mess with the ribosome[J]. Nat Struct Mol Biol, 2006, 13(4):299-301.
[18] CHENG W, CHEN G, JIA H, et al. DDX5 RNA helicases:Emerging roles in viral infection[J]. Int J Mol Sci, 2018, 19(4).
[19] KOKOLO M, BACH-ELIAS M. Downregulation of p68 RNA Helicase (DDX5) activates a survival pathway involving mTOR and MDM2 signals[J]. Folia Biol (Praha), 2017, 63(2):52-59.
[20] MA Z, FENG J, GUO Y, et al. Knockdown of DDX5 inhibits the proliferation and tumorigenesis in esophageal cancer[J]. Oncol Res, 2017, 25(6):887-895.
[21] O'DONNELL J P, MARSH H M, SONDERMANN H, et al. Disrupted hydrogen-bond network and impaired ATPase activity in an Hsc70 cysteine mutant[J]. Biochemistry, 2018, 57(7):1073-1086.
[22] DONG Q, MEN R, DAN X, et al. Hsc70 regulates the IRES activity and serves as an antiviral target of enterovirus A71 infection[J]. Antiviral Res, 2017, 150:39-46.
[23] CHASE A R, LAUDERMILCH E, WANG J, et al. Dynamic functional assembly of the Torsin AAA+ ATPase and its modulation by LAP1[J]. Mol Biol Cell, 2017, 28(21):2765-2772.
[24] ZHOU R, PARK J W, CHUN R F, et al. Concerted effects of heterogeneous nuclear ribonucleoprotein C1/C2 to control vitamin D-directed gene transcription and RNA splicing in human bone cells[J]. Nucleic Acids Res, 2017, 45(2):606-618.
[25] DECHTAWEWAT T SONGPRAKHON P, LIMJINDAPORN T, et al. Role of human heterogeneous nuclear ribonucleoprotein C1/C2 in dengue virus replication[J]. Virol J, 2015, 12(1):14.
[26] MCCLOSKEY A, TANIGUCHI I, SHINMYOZU K, et al. hnRNP C tetramer measures RNA length to classify RNA polymerase Ⅱ transcripts for export[J]. Science, 2012, 335(6076):1643-1646.
[27] SAKONO M, KIDANI T. ATP-independent inhibition of amyloid beta fibrillation by the endoplasmic reticulum resident molecular chaperone GRP78[J]. Biochem Biophys Res Commun, 2017, 493(1):500-503.
[28] SUN C, HAN C, JIANG Y, et al. Inhibition of GRP78 abrogates radioresistance in oropharyngeal carcinoma cells after EGFR inhibition by cetuximab[J]. PLoS One, 2017, 12(12):e0188932.
[29] ZHANG L, LI Z, DING G, et al. GRP78 plays an integral role in tumor cell inflammation-related migration induced by M2 macrophages[J]. Cell Signal, 2017, 37:136-148.
[30] HETTINGHOUSE A, LIU R H, LIU C J. Multifunctional molecule ERp57:From cancer to neurodegenerative diseases[J]. Pharmacol Ther, 2017, 181:34-48.
[31] LIU M, DU L, HE Z, et al. Increased ERp57 expression in HBV-related hepatocellular carcinoma:Possible correlation and prognosis[J]. Biomed Res Int, 2017, 2017(12):1-8.
[32] WANG I H, BURCKHARDT C J, YAKIMOVICH A, et al. The nuclear export factor CRM1 controls juxta-nuclear microtubule-dependent virus transport[J]. J Cell Sci, 2017,130(13):2185-2195.
[33] CHEN Y, CAMACHO S C, SILVERS T R, et al. Inhibition of the nuclear export receptor XPO1 as a therapeutic target for platinum-resistant ovarian cancer[J]. Clin Cancer Res, 2017, 23(6):1552-1563.
[34] CHUTIWITOONCHAI N, MANO T, KAKISAKA M, et al. Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development[J]. Virology, 2017, 507:32-39.
[35] ARTS G J, FORNEROD M, MATTAJ I W. Identification of a nuclear export receptor for tRNA[J]. Curr Biol, 1998, 8(6):305-314.
[36] ARTS G J, KUERSTEN S, ROMBY P, et al. The role of exportin-t in selective nuclear export of mature tRNAs[J]. EMBO J, 2014, 17(24):7430-7441.
[37] GELPERIN D, HORTON L, BECKMAN J, et al. Bms1p, a novel GTP-binding protein, and the related Tsr1p are required for distinct steps of 40S ribosome biogenesis in yeast[J]. RNA, 2001, 7(9):1268-1283.

Transcriptome Reveals the Adapting Mechanism of Colon Tissues of Bactrian Camel in Water-deficient Environment

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