哺乳动物RNA编辑及其检测方法

doi:10.11843/j.issn.0366-6964.2018.11.001

Abstract

Abstract:

RNA editing is an important post-transcriptional regulation mechanism and alter the genetic information by inserting, deleting or replacing bases in primary transcripts. RNA editing can result in amino acid change, alternative splicing, intron retention, modulation of RNA stability, and so on, which will provide a direction for explaining many complex life processes. In mammals, the deamination of adenosine (A) to inosine (I) mediated by adenosine deaminases acting on double-stranded RNA is the most common form of editing. With the development of high-throughput sequencing technology and molecular biology, researchers have developed multiple efficient, flexible and easy-to-use tools to identify the RNA editing sites at the genome-wide level, which lay a foundation for exploring the effects of RNA editing on economic traits of livestock. In this paper, we briefly review the definition, function and detection methods of RNA editing, and introduce some of the most recent and popular detection tools in detail, aiming to draw more attention to RNA editing in livestock.

Key words: high-throughput sequencing, detection methods, RNA editing

CLC Number:

S811.3

ZHANG Yue-bo, YAN Hua, WANG Li-gang, ZHAO Fu-ping, HOU Xin-hua, LIU Xin, GAO Hong-mei, ZHANG Long-chao, WANG Li-xian. RNA Editing and Its Detection Methods in Mammalian[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2299-2309.

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RNA Editing and Its Detection Methods in Mammalian

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