葡萄糖对绒山羊精子冷冻保存及代谢的影响

doi:10.11843/j.issn.0366-6964.2018.09.027

Abstract

Abstract:

The purpose of this study was to investigate the effect of glucose on cryopreservation and metabolism of cashmere goats semen. In this study, the mixed semen samples were diluted in extender containing different concentrations of glucose (0, 28, 56, 84 and 112 mmol·L^-1) and frozen-thawed. After thawing, sperm motility, the percentage of sperm with intact DNA, intact acrosome and intact plasm membrane, level of ROS and Ca²⁺ in sperm and concentration of ATP in sperm, and concentrations of lactic acid and pyruvic acid in sperm suspension were assessed by using computer-assisted sperm analysis system (CASAS), flow cytometry and microplate reader, respectively. The results showed that the semen extender supplemented with 56 mmol·L^-1 glucose could increase sperm motility, the percentage of intact acrosome and plasm membrane in frozen-thaw cashmere goats semen (P<0.05), however no significant differences were observed in the percentages of intact DNA among the groups (P>0.05). The group of 56 mmol·L^-1 glucose could extremely significantly increase the level of ROS (P<0.01),while the groups of 28 and 56 mmol·L^-1 glucose could extremely significantly decrease the level of Ca²⁺(P<0.01); the group of 56 and 84 mmol·L^-1 glucose extremely significantly increase the ATP concentration, in comparison to the other concentration groups (P<0.01). After thawing, lactic acid was not detected in the low concentration glucose groups (28-56 mmol·L^-1), but a large amount of lactic acid was detected in the 112 mmol·L^-1glucose group(P<0.01). Glucose had a dose-dependent effect on pyruvic acid production in sperm suspension when semen extender supplemented with low concentration glucose(28-84 mmol·L^-1), but high concentration glucose (112 mmol·L^-1) significantly decreased pyruvic acid production(P<0.05). In summary, the semen extender supplemented with 56 mmol·L^-1 glucose could promote metabolism of cashmere goat sperm during freeze-thawing and improve cryopreservation effect.

Key words: cashmere goat, sperm, cryopreservation, glucose, lactic acid

CLC Number:

S827.3⁺4

LI Rui-lan, ZHANG Tong, LIU Zhi-hong, WANG Rui-jun, LI Jin-quan, ZHANG Jia-xin. The Effect of Glucose on Cryopreservation and Metabolism of Cashmere Goat Sperm[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2054-2062.

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The Effect of Glucose on Cryopreservation and Metabolism of Cashmere Goat Sperm

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