郁金散对大肠湿热证大鼠血清免疫球蛋白与抗氧化相关因子的调节作用

doi:10.11843/j.issn.0366-6964.2018.09.026

Abstract

Abstract:

This study was conducted to elucidate the regulation of Yujin Powder (YJP) on serum immunoglobulin and antioxidance-related factors of Large Intestine Dampness-heat Syndrome (LIDHS) rat. Sixty Wistar rats were randomly divided into 6 groups with 10 rats in each group:normal control group, LIDHS model group, self-healing group, high, middle and low dose of YJP groups. The rat model of LIDHS was established by such complex factors as high-sugar and high-fat diet, improper diet, drinking, high temperature and humidity environment and intrap-eritoneal injection of Escherichia coli, which imitated the inducing conditions of LIDHS. After the model being successfully established, the rats were treated for 5 days with high, middle and low dose of YJP, respectively. And then levels of serum immunoglobulin IgA, IgG, IgM were detected, the spleen and thymus were collected for histopathological observation, and the lipid peroxide (LPO), malondialdehyde (MDA), reduced glutathione (GSH), and oxide nitrogen (NO) levels in serum and liver tissue homogenate were detected. The results showed that compared with the normal control group, the serum levels of IgA, IgG and IgM in the model group were significantly increased (P<0.01). In comparison with the self-healing group, they were all reversed to different degrees after different doses of YJP administration, and the most obviously in the high-dose group. In high dose group, the content of IgA and IgM were significantly decreased (P<0.05) and IgG was extremely significantly decreased (P<0.01). The pathological observation showed that the splenic and thymic morphological structure of model group rat disorder, spleen congestion, and severe thymus atrophy. In the high-dose group of YJP, the spleen structure tends to be normal, and the degree of thymus atrophy was reduced. Compared with the normal control group, the content of MDA and LPO in the serum and liver tissues in the model group were extremely significantly increased (P<0.01), and the content of GSH and NO were extremely significantly decreased (P<0.01). Compared with the self-healing group, they were all reversed to different degrees after treatment with different doses of YJP administration, and the most obviously in the high-dose group. In high dose group, the content of MDA and LPO were significantly or extremely significantly decreased (P<0.05 or P<0.01), and the GSH and NO content were significantly or extremely significantly increased (P<0.05 or P<0.01). There were abnormal immune function and disturbance of oxidation and antioxidant balance in LIDHS model rats. YJP can treat LIDHS through reducing the serum immunoglobulin levels, improving the structure of spleen and thymus and regulating the balance of oxidation and antioxidation. And the effect of high dose was the best.

Key words: Large Intestine Dampness-heat Syndrome (LIDHS), Yujin Powder, immune, antioxidance

CLC Number:

S853.3

ZHANG Ya-hui, YAO Wan-ling, WEN Yan-qiao, HUA Yong-li, JI Peng, WEI Yan-ming. Regulation of Yujin Powder on Serum Immunoglobulin and Antioxidant Related Factors of Large Intestine Dampness-heat Syndrome Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2044-2053.

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Regulation of Yujin Powder on Serum Immunoglobulin and Antioxidant Related Factors of Large Intestine Dampness-heat Syndrome Rat

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