两株小鼠致病性差异显著的H5N1禽流感病毒PA蛋白差异互作宿主蛋白研究

doi:10.11843/j.issn.0366-6964.2018.09.016

Abstract

Abstract:

To explore the host factors that contribute to the pathogenicity of the H5N1 influenza virus, Two H5N1 avian influenza viruses with different pathogenicity mainly determined by PA (CK10 and GS10) were used in this study. A549 cells were infected with the two viruses and immunoprecipitation (IP) experiments were performed by using PA antibodies subsequently to screen host proteins that interact with PA. The host proteins were further identified by LC-MS/MS. As a result, compared with the low pathogenic GS10, there were 160 host proteins interacting with the high pathogenic CK10 specifically. Bioinformatics analysis was employed targeting on these 160 proteins subsequently. Gene Ontology (GO) annotation analysis showed that these proteins mainly involved in the biological processes of translation, gene expression, viral transcription and viral infection. KEGG pathway analysis revealed that these proteins mainly participate in the cell pathways of translation, infectious diseases and signal transduction. Using co-immunoprecipitation (Co-IP), host protein eEF1A1 was identified to interact with CK10 PA protein specifically. Therefore, this study successfully screened out the differential interactome data of PA protein from different pathogenicity H5N1 influenza viruses. It may play pivotal roles in elucidating the complicated pathogenesis of H5N1 influenza virus.

Key words: H5N1 avian influenza virus, different pathogenicity, host, protein interaction

CLC Number:

S852.659.5

GAO Zhao, HU Jiao, LIU Xiu-fan. Study on the Different Interactions between PA Protein of Two H5N1 Avian Influenza Viruses with Markedly Different Pathogenicity in Mice and Host Proteins[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1952-1960.

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Study on the Different Interactions between PA Protein of Two H5N1 Avian Influenza Viruses with Markedly Different Pathogenicity in Mice and Host Proteins

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