添加不同形式亚麻油对肉羊生产性能、肉品质、脂肪酸含量及脂代谢相关酶基因mRNA表达量的影响

doi:10.11843/j.issn.0366-6964.2018.09.014

Abstract

Abstract:

The aim of this study was to investigate the effects of different forms of linseed oil on the growth performance, meat quality, fatty acid content in muscle and mRNA abundance of lipid metabolism key regulation factors and lipid metabolism related enzymes in sheep. A single-factor experimental design was used. Twenty-four healthy Dorper(♂)×Small-Tailed Han sheep(♀) F₂ male lambs with similar days of age((100±10) d) and body weight((27±2.17) kg) were allocated into 4 groups with 6 sheep in each group. The lambs in control(CON) group were fed with basal diet, the lambs in experimental groups were fed the diets with the same fat content(4%) of linseed oil(LO), linseed(L), linseed oil microcapsule(LOM), respectively. The growth performance indexes such as body weight and dry matter intake of sheep were recorded and the meat quality indexes such as pH and shear force were measured after slaughter. The contents of dry matter, protein, fat and ash in meat were measured by drying, Kjeldahl nitrogen method, Soxhlet extraction and high temperature ash, in addition, the content of fatty acids and the expression of mRNA of lipid metabolism related regulation factors and enzymes in meat were determined by gas chromatography and real-time quantitative PCR. The results showed that:1)The different forms of linseed oil had an effect on sheep ADG, and the ADG of LO group was significantly lower than that of CON, LOM and L groups(P<0.05), the fat content in muscle of the test groups were significantly higher than that of the control group(P<0.05), there was no significant change in other nutritional components in muscle(P>0.05), compared with CON group, muscle shearing force was significantly decreased and tenderness was significantly increased in LOM group (P<0.05), however, the different forms of linseed oil had no significant effect on pH, dripping loss, cooking loss and water loss rate of muscle(P>0.05). 2) Compared with CON group, the addition of different forms of linseed oil significantly increased the content of ALA, CLA and AA in muscle, and significantly improved the ratio of PUFA n-6/n-3(P<0.05), the CLA content of LOM group was significantly increased by 375%, 171% and 58% compared with CON, LO and L groups (P<0.05), and the n-3 PUFA content of L and LOM groups were significantly increased by 151% and 178% compared with CON group(P<0.05). 3) The addition of different forms of linseed oil in diets significantly affected the PPARγ and SREBP1 mRNA expression, compared with group CON, LO, LOM and L groups could increase the expression of PPARγ mRNA by 53%, 68% and 36%, respectively, and down-regulated the expression of SREBP1 mRNA by 47%, 49%, 53%(P<0.05). The addition of linseed oil in each group inhibited the expression of FAS and SCD genes, the expression levels of SCD in LOM group were significantly higher than that in L group(P<0.05), and significantly increased the expression of mRNA of LPL gene, which was 2.7 times higher than that in CON group(P<0.05), but had no significant effect on ACC expression(P>0.05). The addition of linseed oil is beneficial to the deposition of PUFA, promote the expression of PPARγ, LPL genes, and inhibit the expression of SREBP-1, SCO and FAS genes. Linseed oil microcapsule can increase the content of PUFA in muscle, especially the content of CLA, improve the meat quality, increase muscle tenderness, its effect is better in increasing the content of PUFA in muscle of mutton sheep.

Key words: mutton sheep, linseed oil, microcapsule, fatty acids, gene expression

CLC Number:

S826.5

ZHANG Qiu-xu, ZHANG Run-hou, SHI Xiao-xue, LI Gang, GAO Ai-qin. Effects of Supplementation of Different Forms of Linseed Oil on Growth Performance, Meat Quality,Fatty Acid Content and mRNA Abundance of Lipid Metabolism Related Enzymes in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1928-1939.

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Effects of Supplementation of Different Forms of Linseed Oil on Growth Performance, Meat Quality,Fatty Acid Content and mRNA Abundance of Lipid Metabolism Related Enzymes in Sheep

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