FSH对牦牛卵母细胞EGF、EGFR表达及其细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2018.09.011

Abstract

Abstract:

The aim of this study was to investigate the specific contribution of follicle-stimulating hormone (FSH) on maturation of yak oocytes in vitro, the effect of FSH on the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in yak oocytes, and the relationship between FSH and apoptosis. In this experiment, different concentrations of FSH (final concentration:0,2,5,10 μg·mL^-1) were added into the medium of the yak oocytes in vitro. The mRNA and protein expression of EGF and EGFR were detected by real-time PCR (qRT-RCR) and immunofluorescence staining, respectively. Meanwhile, the expression of Bax and Bcl-2 were detected by qRT-RCR and Western blotting. The results showed that:1) It was noteworthy that FSH promoted the maturation of oocytes, and the optimal concentration of FSH was 5 μg·mL^-1, and the rate of maturation oocytes was 76.84%, which was significantly higher than other groups (P<0.05). 2) The expression levels of EGF and EGFR depended on the doses of FSH. The expressions of EGF and EGFR were upregulated during the doses of FSH raised from 0 to 5 μg·mL^-1, which was significantly higher in 5 μg·mL^-1 FSH group than other groups (P<0.05). When the concentration of FSH was 10 μg·mL^-1, the expression level of EGF and EGFR was reduced. Immunofluorescence assay showed that EGF was mainly located in cumulus cells, while EGFR was expressed in cumulus cells and oocytes. 3) With the increase of FSH concentration, the expression of Bax and Bcl-2 showed the obvious reverse pattern, the expression level of Bcl-2 increased gradually, while the expression level of Bax decreased gradually. The expression of Bax was the lowest, and the expression of Bcl-2 was the highest when the dose of FSH was 5 μg·mL^-1, but the expression level of Bax increased in 10 μg·mL^-1 FSH group. The results showed that FSH increased the ability of oocyte development and induced the expression of EGF and EGFR during the process of yak oocyte maturation in vitro, and inhibited the apoptosis of oocytes by regulating the expression of apoptosis related factors such as Bax and Bcl-2.

Key words: yak, oocyte, FSH, EGF, EGFR, apoptosis

CLC Number:

HE Hong-hong, PAN Yang-yang, ZHANG Hui-zhu, LI Qin, WANG Meng, CUI Yan, FAN Jiang-feng, YU Si-jiu. The Effects of Follicle-stimulating Hormone (FSH) on the Expression of EGF and EGFR in Yak Oocytes and Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1899-1907.

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The Effects of Follicle-stimulating Hormone (FSH) on the Expression of EGF and EGFR in Yak Oocytes and Apoptosis

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