ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (9): 1851-1860.doi: 10.11843/j.issn.0366-6964.2018.09.006

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The Expression Patterns of TLRs Gene Family during Duck Bursa of Fabricius Development and Their Molecular Evolutionary Analysis

GAN Xin-meng, CHEN Kai-wen, LIU He-he*, LI Yan-ying, WANG Ji-wen, LI Liang, BAI Li-li   

  1. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2017-11-20 Online:2018-09-23 Published:2018-09-26

Abstract:

This study aimed to explore the expression and function of TLRs gene family in duck bursa of Fabricius, therefore, the mRNA expressions of TLRs genes in duck bursa of Fabricius during the postembryonic development stage were detected by qRT-PCR. The expression patterns were analyzed by clustering and the phylogenetic trees based on the TLRs promoters and coding regions were constructed, respectively. The results showed that the expression level of TLR2a in duck bursa of Fabricius was significantly higher in W2 and W6 than in W4(P<0.05). The expressions of other TLRs in the different postembryonic development stages of duck bursa of Fabricius were not significantly different (P>0.05). TLR1a, TLR2a and TLR2b had similar expression patterns, as well as TLR3, TLR5 and TLR15. In the phylogenetic tree of the coding region, TLR1a, TLR1b, TLR2a and TLR2b were clustered together, and they were closer to TLR15. TLR3 and TLR5 were on the same branch, TLR4 and TLR21 were on two separate branches, respectively, and TLR21 was far away from the other TLRs. However, there was no obvious rules in the phylogenetic tree of TLRs gene family promoter regions. The results indicate that TLRs family members may not be involved in the regulation of postembryonic development in duck bursa of Fabricius; TLR2a and TLR2b may have similar function, as well as TLR3, TLR5 and TLR15. Although TLR1a and TLR1b have high sequence similarity, functional differentiation may occur during the long process of evolution. There was no significant relationship between TLRs expression patterns and its promoter region sequence evolution.

Key words: TLRs, duck, expression pattern, phylogenetic tree

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