猪lncRNA-ENSSSCT00000018610的克隆及其在猪卵泡中的表达

doi:10.11843/j.issn.0366-6964.2018.09.004

Abstract

Abstract:

To explore the role of lncRNA-ENSSSCT00000018610 in the development of porcine follicles, the full length RNA sequence of lncRNA-ENSSSCT00000018610 was amplified by RACE technique, it's tissue expression profile and expression pattern in ovarian follicles of Meishan and Duroc sows were analyzed by Real-time PCR, and it's target genes were predicted by using the cis and trans methods. A 1 731 bp-length sequence was obtained as the full length RNA sequence of lncRNA-ENSSSCT00000018610. lncRNA-ENSSSCT00000018610 was expressed differentially in variety of tissues(muscle, heart, liver, spleen, lung, kidney, duodenum, ovary, fallopian tube, uterus, pituitary, corpus luteum, hypothalamus), and expressed relatively higher in kidney, lung, uterus and ovary. The expression level of lncRNA-ENSSSCT00000018610 in S and L follicles of Duroc were significantly higher than that of Meishan (P<0.05). The expression level of lncRNA-ENSSSCT00000018610 in M1 and M2 follicles of Duroc were significantly higher than that of Meishan (P<0.01). The potential target genes of lncRNA-ENSSSCT00000018610 including TNIP1, CYP2J2, SCARB1 and IBSP were related to the reproduction traits and participated in follicular development of pig. This result suggests that lncRNA-ENSSSCT00000018610 may participate in follicular development indirectly by regulating its target genes expression.

Key words: lncRNA-ENSSSCT00000018610, follicle, expression, sow

CLC Number:

S828.2

LI Meng-xun, HUANG Tao, MA Li-peng, LIU Yi, LI Tao, GONG Hong-bin, QIU Mei-yu, XIE Su, SUN Xiao-mei. Cloning of Porcine lncRNA-ENSSSCT00000018610 and Its Expression Pattern in Porcine Ovarian Follicles[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1830-1839.

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Cloning of Porcine lncRNA-ENSSSCT00000018610 and Its Expression Pattern in Porcine Ovarian Follicles

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