LRP6和VEGFR2在牦牛肺的分布

doi:10.11843/j.issn.0366-6964.2018.07.026

Abstract

Abstract:

This experiment was conducted to investigate the expression and localization of LRP6 and VEGFR2 in yak lungs of different ages and their roles in yak lung hypoxia adaptive structure formation and the possible regulation. Newborn(1-7 days old, n=5), juvenile(5-6 months old, n=5), adult(3-6 years old, n=5) and old(7-10 years old, n=5) yaks were selected as the subjects, the expression and percise location of LRP6 and VEGFR2 in yak lung tissue of different age groups were detected by ELISA, Western-blot and immunohistochemistry. The results showed that the expression of LRP6 and VEGFR2 between the newborn and juvenile yak groups was not significant different(P>0.05); the expression between newborn, juvenile and old yak groups was significant different(P<0.05); the expression between the adult and old yak groups was significant different(P<0.05). The LRP6 was mainly distributed in the lungs of branches and their branches epithelial cells and alveolar type Ⅱ cells and the expression was stronger; the expression in pulmonary vascular endothelial cells and vascular wall smooth muscle cells was weak. The VEGFR2 was mainly distributed in the epithelial cells of the bronchial lung and its branches, wall smooth muscle cells and alveolar septum and alveolar typeⅠcells and the expression was stronger; the expression in pulmonary vascular endothelial cells and smooth muscle cells was weak. The results suggested that LRP6 and VEGFR2 not only had different expression levels in yak lung tissues of different ages, but also had important implications for the regulation of the formation and maintenance of hypoxic adaptative structures in the lungs of yak in the plateau environment.

Key words: yak, lung, LRP6, VEGFR2, adaptability

CLC Number:

S823.8⁺5.2

MA Jun-xing, HE Jun-feng, CUI Yan, ZHANG Qian, LIU Peng-gang. The Distribution of LRP6 and VEGFR2 in Yak Lungs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1550-1557.

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The Distribution of LRP6 and VEGFR2 in Yak Lungs

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