三种多糖对鸡胚肝细胞抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2018.07.022

Abstract

Abstract:

In order to develop the antioxidative polysaccharide preparations, the effects of Lycum barbarum polysaccharide(LBP), Atractylodes macrocephala polysaccharide(AMP) and Chinese Angelica polysaccharide(CAP), on resisting oxidative damage ability of Chicken embryo liver cells(CEL) were compared, vitamin C(VC) were used as control. The safe concentrations of four drugs on CEL were determined. Then they at three concentrations within safe concentrations were respectively added into cultivation system of CEL. After cultivation of 24 hours, the cells were attacked by H₂O₂ for an hour, the cellular vitality(A_{570 nm} value), intra-cellular ROS content, GSH-Px and SOD activities were assayed. The result showed that the A_{570 nm} values in H₂O₂ group were the lowest, ROS contents were the highest and the activities of GSH-Px and SOD were the lowest, which there were significant differences as compared with cell control group(P<0.05). The A_{570 nm} values in 3 polysaccharides and VC groups were higher or significantly higher, ROS contents were lower or significantly lower and the activities of GSH-Px and SOD were higher or significantly higher than those in H₂O₂ group, especially in high concentrations(P<0.05), the actions were most significant. The actions of LBP at 3 concentrations were stronger than those of AMP and CAP at same concentration, and equivalent even superior to VC. The results indicate that three kinds of polysaccharides all possess stronger antioxidant activity, can resist the CEL vitality depress, intra-cellular ROS contents elevation and antioxidase activities decline caused by H₂O₂ attack, thus strengthen CEL ability of oxidation resistance, LBP has the strongest activity and can be considered as polysaccharid antioxidant.

Key words: polysaccharide, chicken embryo liver cells, antioxidative ability

CLC Number:

S853.7

TIAN Wei-jun, QIN Jia-ling, YUE Chan-juan, HOU Ran-ran, LIU Kuan-hui, HU Yuan-liang. Effects of Three Kinds of Polysaccharides on Antioxidative Ability of Chicken Embryo Liver Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1517-1523.

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Effects of Three Kinds of Polysaccharides on Antioxidative Ability of Chicken Embryo Liver Cells

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