牛病毒性腹泻病毒的RT-PCR检测及感染犊牛相关基因差异表达分析

doi:10.11843/j.issn.0366-6964.2018.07.012

Abstract

Abstract:

To explore molecular markers of anti bovine viral diarrhea virus(BVDV) infection in dairy calves, bovine SUMOs(SUMO1, SUMO2, SUMO3, UBC9) and CD4 were selected as related candidate genes, and their differential expression status between BVDV infected and uninfected Holstein calves were detected and analyzed. Blood samples of 42 Holstein calves(Within 2 months old) were collected and whether they carried BVDV or not were identified by specific nested PCR and sequencing techniques. According to the analyses of the nested PCR and sequencing, the calves were divided into two groups(BVDV-carried and un-carried groups) to identify differentially expressed candidate genes of resistance BVDV by fluorescence quantitative PCR. The results showed that 13 cattle of the 42 Holstein calves carried BVDV-1(positive infection rate is 30.9%); the homologies of these 5' UTR reach 95.80% by the analysis of DNAMAN software; compared with the un-carried group, there were significant decreases(P<0.05) in the BVDV-carried calves for the expression of SUMO1, SUMO2 and SUMO3 genes. But there was no significant difference between the two groups for the expression of UBC9 and CD4. Only BVDV-1 was detected in the Holstein calves, which is consistent with the epidemic trend of BVDV in China. The results suggest that there may have a close relationship between BVDV-1 infection and sumoylation, SUMO1, SUMO2 and SUMO3 could be served as candidate genes of bovine BVDV resistance.

Key words: calf diarrhea, bovine viral diarrhea virus, SUMOs, UBC9, CD4

CLC Number:

MI Si-yuan, SHI Ke-rong, ZHANG Rui-qiang, ZHANG Hai-liang, XU Qing-lei, YU Ying. RT-PCR Detection of Bovine Viral Diarrhea Virus Subtypes and Analysis of Differentially Expressed Candidate Genes in Infected Calves[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1432-1439.

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RT-PCR Detection of Bovine Viral Diarrhea Virus Subtypes and Analysis of Differentially Expressed Candidate Genes in Infected Calves

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