外源性添加磷酸二羟基丙酮对小鼠脂肪细胞三酰甘油及关键代谢酶含量的影响

doi:10.11843/j.issn.0366-6964.2018.07.011

Abstract

Abstract:

The purpose of this study was to investigate the effect of exogenously added dihydroxyacetone phosphate on the contents of triglyceride and its key metabolic enzymes in mouse adipocytes. The inoculated mouse 3T3-L1 cell into mature adipocytes were the research object, the cells were cultured in dihydroxyacetone phosphate complete medium containing 0 μmol·L^-1(ck group), 20 μmol·L^-1(group Ⅰ), 50 μmol·L^-1(group Ⅱ) and 200 μmol·L^-1(group Ⅲ) on the 14^th day, and the cells were collected at 0,36 and 72 h for the detection of ELISA and other substances.The contents of key enzymes in triglyceride metabolism were detected by using double antibody sandwich technique,such as acetyl-CoA carboxylase(ACC),fatty acid synthase(FAS), hormone-sensitive lipase(HSL) and other enzymes. The results showed that:1)The contents of triglyceride in the group Ⅰ,Ⅱ and Ⅲ were significantly higher than that in ck group(P<0.01) at 36 and 72 h; The contents of glucose-6-phosphate dehydrogenase(G6PD), dihydrolipoyl transacetylase(DLAT), dihydrolipoyl dehydrogenase(DLD) and FAS in the group Ⅰ,Ⅱ and Ⅲ were significantly higher than that in ck group(P<0.01) at 36 and 72 h; 2) The contents of HSL, carnitine acyltransferase1(CPT1) and free fatty acid(FFA) in the group Ⅰ,Ⅱ and Ⅲ were significantly lower than that in ck group(P<0.01) at 36 and 72 h. The dihydroxyacetone phosphate can promote the synthesis and deposition of triglyceride in mouse adipocytes;The dihydroxyacetone phosphate can also significantly increase the contents of G6PD, DLAT, DLD and FAS in triglyceride synthesis, and can significantly reduce the contents of HSL and CPT1 in triglycerides catabolism.

Key words: dihydroxyacetone phosphate, mouse adipocytes, triglyceride, adipose metabolism

CLC Number:

S816.11

REN Wan-ping, SHAO Wei, LUO Cheng-long, YU Xiong. Effects of Exogenously Added Dihydroxyacetone Phosphate on the Contents of Triglycerides and Key Metabolic Enzymes in Mouse Adipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1423-1431.

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Effects of Exogenously Added Dihydroxyacetone Phosphate on the Contents of Triglycerides and Key Metabolic Enzymes in Mouse Adipocytes

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