ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (6): 1291-1298.doi: 10.11843/j.issn.0366-6964.2018.06.022

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Construction and Biological Characteristics of Salmonella Enterica Serovar Enteritidis luxS Mutant

YU Yan-chao1, WANG Li-li2, PAN Qiao2, XIN Jiu-qing2, WANG Xiu-mei2*   

  1. 1. Animal Science and Technology College, Jilin Agricultural University, Changchun 130118, China;
    2. Animal Mycoplasma Innovation Team, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
  • Received:2017-11-14 Online:2018-06-23 Published:2018-06-22

Abstract:

The study was conducted to investigate the effect of LuxS/AI-2 quorum-sensing system on the biological characteristics of Salmonella enterica Serovar Enteritidis. A luxS mutant strain was constructed by Red homologous recombination system, and its ΔluxS gene stability, growth features, antimicrobial susceptibility, adherence and invasion, as well as biofilm formation were determined and compared with the wild type. Our results showed that the ΔluxS gene exhibits stability in ΔluxS mutant. The growth of ΔluxS mutant was slightly faster than the wild strain in vitro. Biofilm formation of the mutant strain was significantly enhanced as well as the adhesion and invasion ability to DF-1 cells and Caco-2 cells. Deletion of luxS resulted in a 128-to 256-fold increase in susceptibility to fluoroquinolones and moderate increases (2-to 8-fold) in susceptibility to ampicillin, tetracycline, doxycycline, chloramphenicol, and florfenicol. These results suggest that LuxS/AI-2 system affects biological characteristics of SE including biofilm formation, adhesion and invasion to cells, moreover, the AI-2/LuxS sensing system affect antimicrobial susceptibility of SE through certain resistance mechanisms.

Key words: Salmonella enterica Serovar Enteritidis, quorum sensing system, luxS gene, antimicrobial susceptibility

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