猪Beclin1基因调控伪狂犬病病毒诱导细胞自噬和凋亡的机制分析

doi:10.11843/j.issn.0366-6964.2018.06.020

Abstract

Abstract:

To study the relationship of autophagy and apoptosis after PK-15 cells were infected by pseudorabies virus (PRV), porcine host anti-apoptotic family proteins (Bcl-2 and Bcl-xL) interacted with Beclin1 were further analyzed. Western blotting (WB) was taken to measure the expression of LC3 and P62 proteins; and the level of apoptosis in PK-15 cells infected by PRV was investigated by Flow cytometry. Finally, Co-immunoprecipitation (Co-IP) was used to investigate the interaction between Beclin1 and Bcl-2/Bcl-xL. The expression of LC3-Ⅱ significantly increased, while that of P62 and p-AKT (S473) significantly decreased. In addition, the results of Flow cytometry showed that apoptosis was induced by PRV. Meanwhile, cleaved Caspase-9/8 significantly increased compared to control group detected by WB. Moreover, Co-IP results showed that the infection of PRV prevented the association between Beclin1/Bcl-2 and Beclin1/Bcl-xL. PRV induces autophagy which may depend on PI3K-I/AKT and apoptosis which simultaneously active Caspase-9 and Caspase-8, respectively. Autophagy might be induced through the interactions of Beclin1/Bcl-2 or Beclin1/Bcl-xL.

Key words: pseudorabies virus, autophagy, apoptosis, Beclin1

CLC Number:

HU Lin, NAN Liang-kang, ZHANG Min, XU Su-tong, YANG Liao-han, LI Shuai-feng, WANG Kai, LIU Zheng-fei, ZHANG Shu-jun. The Mechanism of Porcine Beclin1 Gene on Autophagy and Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1274-1281.

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The Mechanism of Porcine Beclin1 Gene on Autophagy and Apoptosis

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