广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析

doi:10.11843/j.issn.0366-6964.2018.06.019

Abstract

Abstract:

In order to understand the epidemic status and phylogenetic relationship of porcine circovirus type 3 in healthy slaughter pigs, 181 lymph nodes collected from slaughterhouse in Guangxi were detected for PCV3 pathogens. PCV3 infection in healthy slaughter pigs was detected by PCR method. The positive rate of PCV3 was 24.31%, which suggest that the infection of PCV3 in healthy pigs is more severe. Six PCV3 complete genome sequences were amplified with PCR in the positive samples.Compared with PCV3 complete genome in GenBank and the results showed the nucleotide homology of PCV3 sequences was 98.6%-99.9% and genes of PCV3 were very conservative and similar. Genetic evolution analysis of PCV3 ORF2 gene showed that PCV3 strains could be divided into two major types. Analyses of the phylogenetic relationship of PCVs Cap and suggesting cross protection between PCV2 and PCV3 is impossible.

Key words: porcine circovirus type 3, complete genenome, sequence analysis, phylogenetic analysis

CLC Number:

S852.659.2

JI Cheng-yuan, WANG Wei-yi, FENG Xuan-rong, YAO Jing, HUANG Xin, FENG Yuan-qing, QIN Yi-feng, FANG Qing-li, CHEN Ying, OUYANG Kang, WEI Zu-zhang, HUANG Wei-jian. Epidemiological Investigation and Genetic Diversity Analysis of Porcine Circovirus Type 3 in Slaughter Pigs of a City in Guangxi[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1265-1273.

References

[1] TISCHER I, GELDERBLOM H, VETTERMANN W, et al. A very small porcine virus with circular single-stranded DNA[J]. Nature, 1982,295(5844):64-66.
[2] FRANZO G,TUCCIARONE C M,DOTTO G, et al. International trades, local spread and viral evolution:The case of porcine circovirus type 2(PCV2) strains heterogeneity in Italy[J]. Infect Genet Evol, 2015, 32:409-415.
[3] MOROZOV I, SIRINARUMITR T, SORDEN S D, et al. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome[J].J Clin Microbiol, 1998, 36(9):2535-2541.
[4] MAHÉ D, BLANCHARD P, TRUONG C, et al. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes[J]. J Gen Virol, 2000, 81:1815-1824.
[5] LIU Q, WILLSON P, ATTOH-POKU S, et al. Bacterial expression of an immunologically reactive PCV2 ORF2 fusion protein[J].Protein Expr Purif, 2001, 21(1):115-120.
[6] NAWAGITGUL P, MOROZOV I, BOLIN S R, et al. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein[J]. J Gen Virol, 2000, 81(Pt 9):2281-2287.
[7] TISCHER I, RASCH R, TOCHTERMANN G. Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines[J]. Zentralbl Bakteriol Orig A, 1974, 226(2):153-167.
[8] HARMS P A, SORDEN S D, HALBUR P G, et al. Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus[J].Vet Pathol, 2001, 38(5):528-539.
[9] CHOI C, KIM J, KANG I J, et al. Concurrent outbreak of PMWS and PDNS in a herd of pigs in Korea[J]. Vet Rec, 2002, 151(16):484-485.
[10] HINES R K, LUKERT P D. Porcine circovirus as a cause of congenital tremors in newborn pigs[C]//Proceedings of America Association Swine Prsctitioners. Chicago:America Association Swine Prsctitioners,1994:344-345.
[11] PALINSKI R, PIÑEYRO P, SHANG P C, et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure[J]. J Virol, 2016, 91(1):e01879-16.
[12] GRAU-ROMA L, FRAILE L, SEGALÉS J. Recent advances in the epidemiology,diagnosis and control of diseases caused by porcine circovirus type 2[J].Vet J, 2011, 187(1):23-32.
[13] KU X,CHEN F,LI P,et al.Identification and genetic characterization of porcine circovirus type 3 in China[J]. Transbound Emerg Dis, 2017, 64(3):703-708.
[14] CHEN G H, MAI K J, ZHOU L, et al. Detection and genome sequencing of porcine circovirus 3 in neonatal pigs with congenital tremors in South China[J].Transbound Emerg Dis, 2017, 64(6):1650-1654.
[15] 贺会利, 李军, 潘艳, 等. 广西首例猪圆环病毒3型的发现及其衣壳蛋白序列分析[J].南方农业学报, 2017, 48(8):1499-1503.
HE H L, LI J, PAN Y, et al. The first report of porcine circovirus type 3 infection in Guangxi and sequence analysis of its capsid protein[J].Journal of Southern Agriculture, 2017, 48(8):1499-1503. (in Chinese)

Epidemiological Investigation and Genetic Diversity Analysis of Porcine Circovirus Type 3 in Slaughter Pigs of a City in Guangxi

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