ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (6): 1204-1211.doi: 10.11843/j.issn.0366-6964.2018.06.012

Previous Articles     Next Articles

TPL2 Enhances the Replication of Foot-and-mouth Disease Virus in BHK-21 Cells

WEI Nan-nan, XU Shou-xing, SHI Xi-juan, LU Bing-zhou, ZHANG Ke-shan*, ZHENG Hai-xue*, LIU Xiang-tao   

  1. State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:2017-11-01 Online:2018-06-23 Published:2018-06-22

Abstract:

This experiment was conducted to study the effects of tumor progression locus 2 (TPL2) on replication of foot-and-mouth disease virus (FMDV) in baby hamster kidney (BHK-21) cells. BHK-21 cells were infected by FMDV, the mRNA level of TPL2 was detected by qRT-PCR. TPL2 eukaryotic expression plasmid and RNAi sequences were designed and synthesized based on TPL2 sequences published in GenBank (NM007746.2). The effects of TPL2 on FMDV replication in BHK-21 cells were evaluated by qRT-PCR and Western blotting. The results indicated that the transcriptional level of TPL2 was significantly up-regulated during FMDV infection; Overexpression of TPL2 facilitated the replication of FMDV and downregulation of endogenous TPL2 inhibited FMDV replication. These results revealed that TPL2 promoted the proliferation of FMDV in BHK-21 cells. This research further expanded our understanding of the relationship between FMDV and the innate immune mechanism and had accumulated scientific data for the relevant research of TPL2.

Key words: TPL2, foot-and-mouth disease virus, FMDV, BHK-21 cell, innate immune, replication

CLC Number: