ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (6): 1134-1144.doi: 10.11843/j.issn.0366-6964.2018.06.005

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Identification of microRNAs Involved in Myogenic Differentiation of Bovine Fetal Skeletal Muscle Derived Myoblasts

XING Yi-shen, HU Xin, REN Ling, WANG Ya-hui, XU Ling-yang, LI Jun-ya*, ZHANG Lu-pei*   

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2017-12-13 Online:2018-06-23 Published:2018-06-22

Abstract:

This study aimed to identify differentially expressed miRNAs in myogenic differentiation of bovine fetal skeletal muscle derived myoblasts, which would provide important insight for understanding the mechanism of myoblast differentiation at bovine fetal stage. Myoblasts were isolated and cultured from skeletal muscle of 3 bovine fetal on 4 months old, and then induced myogenic differentiation in vitro. miRNAs of myoblast at the initial and terminal stage of myoblast differentiation were sequenced using high throughput sequencing technology. The sequencing data was analyzed to identify the differentially expressed miRNAs. Differentially expressed miRNAs were randomly selected to validate the sequencing accuracy using quantitative RT-PCR. The results revealed 619 miRNAs in 6 libraries, and 150 miRNAs were differentially expressed. Among them, several miRNAs were related to muscle development such as bta-miR-199a-5p. The target genes of differentially expressed miRNAs were predicted and enriched to pathways related to the muscle development such as MAPK pathway. The expression of 8 randomly selected miRNAs were quantified by real-time quantitative PCR for further validation, and their results were consistent with those of sequencing analyses. The study identified 150 differentially expressed miRNAs in the differentiation of bovine fetal muscle derived myoblasts. Our findings will provide valuable insights for further exploring the regulation roles of miRNAs in the development of bovine fetal skeletal muscle.

Key words: bovine, fetal skeletal muscle, miRNA, myoblast differentiation

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