ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (6): 1108-1115.doi: 10.11843/j.issn.0366-6964.2018.06.002

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Tissue Expression Profiles of HLCS Gene and Its Association with Residual Feed Intake in Duroc

ZHANG Yue-bo1, PU Lei2, ZHANG Jin-shan1, YAN Hua1, WANG Li-gang1, HOU Xin-hua1, LIU Xin1, GAO Hong-mei1, WANG Li-xian1, ZHANG Long-chao1*   

  1. 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2017-12-28 Online:2018-06-23 Published:2018-06-22
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Abstract:

This experiment was conducted to explore the tissue expression profile of HLCS, the polymorphisms in its coding region and the association of different genotypes with residual feed intake in Duroc. Real-time PCR was used to detect HLCS mRNA expression in 8 tissues of Duroc pigs. The polymorphic sites of HLCS were determined in Duroc with high and low RFI lines by PCR and sequencing. Genotypes were detected in Duroc, and their associations with residual feed intake trait were analyzed. Real-time PCR showed that HLCS had the highest expression in backfat, followed by liver, and it had the lowest expression in heart and muscle. Five SNPs were detected in the coding region of HLCS gene. Two of them, c.1408G > C and c.1976A > G were nonsynonymous mutations. The site c.1976A > G was significantly associated with RFI(P<0.05), and the individuals with GG genotype had lower RFI than individuals with AA genotype. These results indicate that HLCS may play an important role in porcine RFI, and c.1976A > G can serve as a useful genetic marker for reducing RFI in swine. This study is valuable to further explore the molecular mechanism of HLCS affecting residual feed intake.

Key words: HLCS gene, RFI, SNP, swine, tissue expression

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