ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (5): 1020-1026.doi: 10.11843/j.issn.0366-6964.2018.05.017

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Immune Efficacy Analysis of Flagellin Protein FliC of Salmonella abortus equi on SeM of Streptococcus equi subsp. equi

ZHAO Ya-nan, YANG Kang, ZHANG Bao-jiang, LI Bin, SU Yan*   

  1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2017-09-20 Online:2018-05-23 Published:2018-05-23

Abstract:

The aim of this study was to investigate the effects of the flagellin protein FliC of Salmonella abortus equi on immune response of Streptococcus equi subsp. equi SeM and provide basis for the development of a new effective strangle vaccine. The recombinant protein FliC was constructed, expressed and purified using affinity chromatography, and its immunogenicity was analyzed by Western blot. The purified FliC and SeM were used in-combination to immunize C57BL/6 mice and the serum antibody level, antibody subtype of immunized mice and challenge protection rate were investigated. SDS-PAGE result indicated that the expressed recombinant FliC protein was 52 kDa. The results of antibody typing showed that the main antibodies were mainly IgG1, and the challenge protection rate of FliC+SeM immunization group (87%) was higher than that of SeM immunization group (67%). The flagellin FliC of Salmonella abortus equi could enhance the immune efficacy of SeM and this result laid experimental foundation for the further utilization of SeM.

Key words: Salmonella abortus equi, FliC, Streptococcus equi subsp.equi, SeM, immune efficacy, immune protection

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