gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭

doi:10.11843/j.issn.0366-6964.2018.05.015

Abstract

Abstract:

Specific pathogen free Leghorn chickens were infected with MDV, and lymphoma from spleens and livers were collected post infection. The qRT-PCR assay showed that microRNA gga-miR-140-3p was downregulated in MDV-induced tumorous spleen and lymphoma from liver. To elucidate the functions of this miRNA in Marek's disease tumor transformation, we transfected MDV-transformed lymphoid cell line, MDCC-MSB1 with gga-miR-140-3p agomir or agomir NC, and inspected their effects on cell proliferation and migration as well as MMP2 and MMP9 genes expression levels whose expression levels were closely correlated with invasion. The proliferation of MSB1 cells decreased at 24, 36, 48, 60 and 72 h post gga-miR-140-3p agomir transfection. The cell number of migration also decreased after transfection with gga-miR-140-3p agomir. MMP2 expression was significantly downregulated at 24, 48 and 72 h; in addition, MMP9 transcription was remarkably downregulated at 48 h post gga-miR-140-3p agomir transfection. In conclusion, gga-miR-140-3p which was downregulated in MDV-induced tumorous tissues had an effect on features of tumor cells such as proliferation, migration and invasion, which indicates that this miRNA may be involved in Marek's disease tumor transformation.

Key words: Marek's disease virus, proliferation, migration, MMP2 gene, MMP9 gene

CLC Number:

ZHAO Chun-fang, LI Xin, HAN Bo, QU Lu-jiang, LIU Chang-jun, SONG Jiu-zhou, LIAN Ling, YANG Ning. gga-miR-140-3p Inhibit Proliferation, Migration and Invasion in MDV-transformed MSB1 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1005-1011.

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gga-miR-140-3p Inhibit Proliferation, Migration and Invasion in MDV-transformed MSB1 Cells

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