槲皮素通过影响Nrf2-Keap1信号通路减缓镉中毒大鼠的生殖机能损伤

doi:10.11843/j.issn.0366-6964.2018.05.019

Abstract

Abstract:

The aim of this study was to investigate the effect of Quercetin (QE) on the reproductive system damage induced by cadmium (Cd) in male rats and the role of Nrf2-Keap1 signaling pathway in this process. A total 32 3-month-old SPF Wistar male rats were randomly divided into four groups with eight rats in each group:control group[0.5 mL·(kg·d)^-1 physiological saline], Cd group[5 mg·(kg·d)^-1 CdCl₂], QE group[15 mg·(kg·d)^-1 QE], Cd+ QE group[5 mg·(kg·d)^-1 CdCl₂+15 mg·(kg·d)^-1 QE], the study lasted for 30 days. At the end of the trial, the testis and epididymis organs coefficients were calculated, then the sperm quality and the levels of anti-oxidation of testis were measured. RT-qPCR and Western blot were used to detect the changes of mRNA and protein expressions of Nrf2 and its related genes, respectively. The results showed that the reproductive function of rats in Cd group was seriously damaged compared with the control group. Compared with Cd group, the coefficients of testis and epididymis (P<0.01) and the secretion testosterone of rats in Cd+QE group were significantly increased (P<0.05). The semen quality was improved, furthermore, the levels of MDA, H₂O₂ were significantly decreased (P<0.01) and the levels of GSH (P<0.05), CAT, T-SOD, GSH-Px (P<0.01) were significantly increased in Cd+QE group. The analysis showed that the mRNA and protein expression levels of Nrf2, HO-1, NQO1, GSH-Px, γ-GCS in testis were significantly increased (P<0.05 or P <0.01) and Keap1 mRNA and protein expression levels were significantly decreased (P <0.05) in Cd+QE group. In conclusion, QE can effectively alleviate the reproductive system damage of male rats induced by Cd through activating Nrf2-Keap1 signaling pathway.

CLC Number:

S856.9

HE Liu, LI Hui, ZHOU Hou-ji, TONG Cui, WANG Jia-mei, LI Mei-qi, YANG Shu-hua, HE Jian-bin. Protective Effects of Quercetin on the Reproductive Injury Induced by Cadmium through the Nrf2-Keap1 Signaling Pathway in Rats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1037-1046.

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Protective Effects of Quercetin on the Reproductive Injury Induced by Cadmium through the Nrf2-Keap1 Signaling Pathway in Rats

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