ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (8): 1045-1049.doi:

• 遗传繁育 • Previous Articles     Next Articles

Studies on in vitro Fertilization of Porcine Frozen Semen

DING Yi1,2,3, DAI Jian-jun1,2,WU Hua-li1,2，ZHANG De-fu1,2*, XU Gang-yi3   

  1. 1.Institute of Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106,China; 2.Shanghai Municipal Key Laboratory of Agrigenetics and Breeding, Shanghai 201106, China;3.College of Animal Science, Sichuan Agricultural University,Ya’an 625014, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-24 Published:2008-08-24
  • Contact: ZHANG De-fu

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Abstract: In order to simplify the source of semen of in vitro fertilization(IVF), and avoid the effects by different boars and different semen collection time, we used frozen semen by 5 mL-straw to do the IVF. The results showed that there were no significant differences in the polyspermy rates among the 2-8 h oocyte-sperm incubation time(P>0.05). The 2-cell rate was improved along with the increasing of oocyte-sperm incubation time, and 8 h group (72.8%) was much higher than the 2 h group(60.5%, P<0.05). The morula and blastocysts rates of the 6 h group were the highest and got 46.1% and 3.48%, respectively. Polyspermy rate was also increased with the increasing of sperm concentration in the IVF (P<0.05). The 2-cell rate of 108 group(81.2%) was the highest, which was much higher than the 105 group. The 107 and 106 group got 8.11% and 3.13% blastocyst rate, respectively. Using NCSU-23 as the in vitro maturation(IVM) medium and matured oocytes partly denuded cumulus cells could get the highest cleavage and blastocyst rates(77.1%,7.33%) among the four groups. The comparation between the frozen semen and fresh semen in the IVF showed that there were no significant differences in the cleavage, morula and blastocyst rates(P>0.05).