绵羊肺腺瘤病毒囊膜蛋白引起绵羊绒毛膜滋养层细胞的恶性转化

doi:10.11843/j.issn.0366-6964.2018.05.025

Abstract

Abstract:

In order to clarify the tumorigenicity of Jaagsiekte sheep retrovirus (JSRV) envelope protein (Env), the recombinant eukaryotic expression plasmids pEGFP-C1/exJSRV-env and pEGFP-C1/enJSRV-env were transfected into immortalized sheep chorionic trophoblast cells (STCs) respectively. The optimal transfection conditions were screened and the soft agar colony forming assay was used to detect the malignant transformation of the cells, and the effect of the expression of the envelope protein on cell proliferation was detected by plate cloning assay. Results were as follows:Sheep trophoblast cells transfected with pEGFP-C1/exJSRV-env acquired colony forming ability in soft agar and lost contact inhibition, while cells transfected with pEGFP-C1/enJSRV-env can't form colony. The results of plate cloning experiments were analyzed by SPSS software, the clone formation rate of sheep trophoblast cells transfected with pEGFP-C1/exJSRV-env was significantly higher than that of transfected with pEGFP-C1/enJSRV-env, pEGFP-C1 empty carrier and non-transfected (P<0.01). The expression of exJSRV-Env can induce malignant transformation and the proliferation of STCs. This study may provide a theoretical basis for further study on the structure and function of Jaagsiekte sheep retrovirus envelope protein, and provide a new idea for the study of the mechanism of sheep pulmonary adenomatosis.

CLC Number:

S852.659.3

ZHAO Juan, XU Si-ri-gu-leng, LI Hui-ping, LIU Shu-ying. Malignant Transformation of Sheep Trophoblast Cells Induced by Envelope Protein of Jaagsiekte Sheep Retrovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1089-1095.

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Malignant Transformation of Sheep Trophoblast Cells Induced by Envelope Protein of Jaagsiekte Sheep Retrovirus

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