ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (5): 1047-1053.doi: 10.11843/j.issn.0366-6964.2018.05.020

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Regulation of RANK/RANKL/OPG Signal Pathway by Calcium Chelated Sheep Bone Collagen Peptide in Inhibiting the Occurrence of Osteoporosis

HAN Ke-guang1, SHEN Yong-tao1, YUAN Kai1, FAN Hua2, CHEN Jing1, JIN Shu-xiu1, GU Shao-peng1, TIAN Wen-xia1, ZHANG Ding1, HUO Nai-rui1*, CHANG Hong3*   

  1. 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    2. Shanxi Agricultural University Experimental Center, Taigu 030801, China;
    3. College of Life Science, Shanxi Agricultural University, Taigu 030801, China
  • Received:2017-10-09 Online:2018-05-23 Published:2018-05-23

Abstract:

OPG/RANKL/RANK signal pathway plays critical roles in the formation of osteoclasts. Inhibitive roles of calcium chelated sheep bone collagen peptide (SBCP-Ca) in the development of osteoporosis (OP) and its mechanism based on the OPG/RANKL/RANK signal pathway were investigated in this study. OP models of SD rats were established by bilateral ovariectomy. SBCP-Ca (100 mg·mL-1) was intragastrically administered at 10 mL·(kg·d)-1 for 8 weeks. After treatment, scanning electron microscopy for metaphysis of distal femur showed that OP models were successfully established. The serum levels of PINP and β-CTx, which reflected bone formation and bone resorption respectively, were significantly higher in model group (P<0.01), while the calcium and proline contents in femur bone were significantly lower than the sham group (P<0.01).The levels of PINP and β-CTx in SBCP-Ca group showed no statistical differences compared with the sham group (P>0.05), both Ca and proline content were higher than that of the model group (P<0.01). The results of real-time PCR suggested that the relative mRNA levels of RANK and RANKL in model group were significantly higher than that of the sham group (P<0.01), while the expression of OPG showed no significant changes. The results showed that the transcriptional levels of RANK and RANKL were significantly lower in SBCP-Ca group than that of the model group(P<0.01), and the OPG level was significantly higher than that of the other groups(P<0.01). It can be concluded that SBCP-Ca could inhibit the occurrence of osteoporosis effectively by inhibiting the expression of RANK and RANKL, and promoting the expression of OPG in bone tissue. The triple actions of SBCP-Ca inhibited the formation and the resorption activity of osteoclasts.

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