成纤维生长因子4在妊娠早期牛胎盘组织的分布及表达特点

doi:10.11843/j.issn.0366-6964.2019.08.018

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (8): 1685-1693.doi: 10.11843/j.issn.0366-6964.2019.08.018

成纤维生长因子4在妊娠早期牛胎盘组织的分布及表达特点

张芮琪, 张莹利, 蒋明, 徐越, 徐永平*, 卿素珠*

西北农林科技大学动物医学院, 杨凌 712100

收稿日期:2019-01-22 出版日期:2019-08-23 发布日期:2019-08-19
通讯作者: 徐永平,主要从事基础兽医学和发育生物学研究,E-mail:xuyp717@nwsuaf.edu.cn;卿素珠,主要从事基础兽医学和发育生物学研究,E-mail:suzhuqing@163.com
作者简介:张芮琪(1994-),女,山西芮城人,硕士生,主要从事动物神经内分泌免疫调控及动物生殖病理研究,E-mail:zhanngruiqi@163.com
基金资助:
国家自然科学基金面上项目（31472094）；陕西省科技攻关项目（2018-NY-001）

Expression of Fibroblast Growth Factor 4 in Bovine Placenta at Early Pregnancy

ZHANG Ruiqi, ZHANG Yingli, JIANG Ming, XU Yue, XU Yongping*, QING Suzhu*

College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China

Received:2019-01-22 Online:2019-08-23 Published:2019-08-19
Supported by:

摘要/Abstract

摘要： 成纤维生长因子4（FGF4）是在哺乳动物胚胎发育过程中第一个被发现的FGF分子，在促进滋养层细胞的增殖方面具有广泛的作用。已经证实，FGF4是小鼠早期胚胎发育过程中的一个重要因子，异常表达会影响早期胚胎的发育，并影响小鼠胎盘的发育及功能。截至目前，有关FGF4在牛胎盘发育过程中的表达尚无报道。本试验运用石蜡切片HE、PAS、免疫荧光染色技术和实时荧光定量PCR（qRT-PCR）技术对妊娠早期牛胎盘组织结构特征及胎盘组织中FGF4的细胞定位和mRNA表达规律进行研究。结果显示，在所检测的各胎龄胎盘的羊膜、子叶及肉阜组织均检测到Fgf4 mRNA，各部位表达量依区域和孕龄而异；在牛胎盘块的单核滋养层细胞、滋养层巨细胞、肉阜上皮、子宫内膜上皮及子宫腺等部位的细胞质均观察到FGF4阳性反应，滋养层巨细胞及子宫腺上皮呈强染色。可见，在妊娠早期的牛胎盘组织存在FGF4及其mRNA，随着胎龄的增加其表达量在胎儿胎盘（子叶）表现为先降低而后增高的规律，而在母体胎盘（肉阜组织）则呈现先逐渐升高然后降低的趋势。

关键词: FGF4, 妊娠早期, 牛胎盘, 表达

Abstract: Fibroblast growth factor 4(FGF4) is the first FGF molecule that discovered during the mammalian embryo development, which plays a key role in promoting proliferation of trophoblast cells. Abnormal expression of FGF4 affects development of early embryos and affects the growth and function of mouse placenta as well. Until now, FGF4 is not identified in bovine placenta at early pregnancy. In this study, paraffin sections were prepared and stained with HE, PAS to study the structure in bovine placenta at early pregnancy. In addition, immunofluorescence staining and qRT-PCR were used to study the cell location and expression of FGF4 in bovine placenta at early pregnancy. The results showed that Fgf4 mRNA was detected in amnion, cotyledon and caruncle at every detected age, and the expression of every part varied according to region and gestational age. FGF4 was detected in the cytoplasm of mononuclear trophoblast, trophoblast giant cell, caruncle epithelia, endometrium epithelia, and uterine gland, and was strongly stained in trophoblast giant cell and uterine glandular epithelia. FGF4 and its mRNA were exist in bovine placenta at early pregnancy. With the growth of gestational age, the relative expression of FGF4 mRNA was plunged at first, and then increased in fetal placenta (cotyledon); and in maternal placenta (caruncle), the expression was firstly increased and then decreased.

Key words: FGF4, early pregnancy, bovine placenta, expression

中图分类号:

S852.16

张芮琪, 张莹利, 蒋明, 徐越, 徐永平, 卿素珠. 成纤维生长因子4在妊娠早期牛胎盘组织的分布及表达特点[J]. 畜牧兽医学报, 2019, 50(8): 1685-1693.

ZHANG Ruiqi, ZHANG Yingli, JIANG Ming, XU Yue, XU Yongping, QING Suzhu. Expression of Fibroblast Growth Factor 4 in Bovine Placenta at Early Pregnancy[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1685-1693.

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成纤维生长因子4在妊娠早期牛胎盘组织的分布及表达特点

Expression of Fibroblast Growth Factor 4 in Bovine Placenta at Early Pregnancy

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