鹅成熟卵泡壁层组织动态发育特点-卵泡发育与分级的新视角

doi:10.11843/j.issn.0366-6964.2019.08.009

摘要/Abstract

摘要： 旨在揭示鹅成熟卵泡壁层中各个结构组分的形态特点与发育规律，为禽类成熟卵泡动态发育的分级提供新的视角与依据。本研究选用健康的高产期天府肉鹅母鹅5只，取其各个阶段（直径0～2、2～4、4～6、6～8、8～10 mm与F5～F1）的成熟卵泡进行固定。在0～10 mm中的每个阶段分别随机选取至少3个卵泡，其中每个卵泡（除0～2 mm阶段）均随机选取3个以上不同区域进行HE切片制作，而F5～F1阶段的每个卵泡则随机选取3个以上不同区域进行HE切片制作。每个切片随机取3个以上的区域进行图像采集，分别在每张图像上随机选取3个区域对其对应的卵泡壁层、颗粒层、膜层与结缔层的组织厚度进行测量、统计与分析，对其形态结构进行比较与观察。结果表明：1）在部分直径2 mm以下的卵泡中，还未形成完整的内膜层。2）直径2～10 mm的卵泡与F5卵泡在卵泡尺寸与卵泡壁层形态上并未表现出明显的差异。3） F4~F2阶段的卵泡中，其颗粒层细胞呈扁平状；而膜层与结缔层厚度显著增加（P<0.05），卵泡壁层整体形态发生了较大的改变。4） F1阶段卵泡的颗粒层为疏松的立方状，且其卵泡壁的各个组分厚度也均出现了显著的变化（P<0.05）。结果提示，从成熟卵泡壁层组织形态和结构分析，鹅卵泡的发育历程可划分为2 mm以下、2～10 mm、F5、F4～F2与F1卵泡5个不同的阶段，这些阶段均有其独特的形态、结构特点，且这样的特点很可能与其功能的差异密切相关。

关键词: 鹅, 成熟卵泡, 卵泡壁层, 发育, 分级

Abstract: The purpose of this study was to reveal the morphological characteristics and developmental regularity of various components in the mature follicle wall layer of goose, and to provide a new perspective for the grading of dynamic development of mature follicles in poultry. The 5 healthy female geese from maternal line of Tianfu geese in high-yielding period were selected, and their mature follicles at each stage (diameter 0-2, 2-4, 4-6, 6-8, 8-10 mm and F5-F1) were fixed. At least 3 follicles were randomly selected in each stage during 0-10 mm, and more than 3 different regions were randomly selected for HE slicing in each follicle (except 0-2 mm stage); While more than 3 different regions were randomly selected for HE slicing in each follicle during F5-F1 stage. More than 3 areas were randomly selected from each slice for image acquisition, and 3 areas were randomly selected from each image to measure. We counted and analyzed the thickness of follicular wall, granulosa layer, theca layer and connective layer, and observed and compared their morphological characteristics. The results showed that:1) There was no complete internal theca layer in some follicles less than 2 mm in diameter. 2) There was no significant difference in follicle size and follicular wall morphology between 2-10 mm and F5 follicles. 3) In stage F4-F2 follicles, the granulosa layer cells were flat, while the thickness of theca and connective layer increased significantly (P<0.05), and the morphology of follicular wall layer changed greatly. 4) The granulosa layer of F1 follicle became loose cubes, and the thickness of each component of follicular wall changed significantly (P<0.05). These results indicate that the development process of goose mature follicle can be divided into 5 stages:0-2 mm, 2-10 mm, F5, F4-F2 and F1. These follicles at different stages have unique morphological and structural characteristics, which may be closely related to their functional differences.

Key words: goose, mature follicle, follicular wall, development, grading

中图分类号:

S835.3

甘翔, 王继文, 李琴, 邓艳, 胡继伟, 李亮, 韩春春. 鹅成熟卵泡壁层组织动态发育特点-卵泡发育与分级的新视角[J]. 畜牧兽医学报, 2019, 50(8): 1607-1613.

GAN Xiang, WANG Jiwen, LI Qin, DENG Yan, HU Jiwei, LI Liang, HAN Chunchun. Dynamic Development Characteristics of Mature Follicular Wall in Goose: A New Perspective of Follicle Development and Grading[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1607-1613.

参考文献 35

[1]	JOHNSON A L,WOOD D C. Ovarian dynamics and follicledevelopment[M]//JAMIESON B G M.Reproductive biology and phylogeny of birds[M].Enfield,NH:Science Publishers,2007.
[2]	LOVELL T M,GLADWELL R T,GROOME N P,et al.Ovarian follicle development in the laying hen is accompanied by divergent changes in inhibin A,inhibin B,activin A and follistatin production in granulosa and theca layers[J].J Endocrinol,2003,177(1):45-55.
[3]	ONAGBESAN O,BRUGGEMAN V,DECUYPERE E.Intra-ovarian growth factors regulating ovarian function in avian species:A review[J].Anim Reprod Sci,2009,111(2-4):121-140.
[4]	WILLIAMS J B,SHARP P J.Ovarian morphology and rates of ovarian follicular development in laying broiler breeders and commercial egg-producing hens[J].Br Poult Sci,1978,19(3):387-395.
[5]	NITTA H,MASON J I,BAHR J M.Localization of 3β-hydroxysteroid dehydrogenase in the chicken ovarian follicle shifts from the theca layer to granulosa layer with follicular maturation[J].Biol Reprod,1993,48(1):110-116.
[6]	HOCKING P M,GILBERT A B,WALKER M,et al.Ovarian follicular structure of White Leghorns fed ad libitum and dwarf and normal broiler breeders fed ad libitum or restricted until point of lay[J].Br Poult Sci,1987,28(3):493-506.
[7]	PEDERSEN T P H.Proposal for a classification of oocytes and follicles in the mouse ovary[J].J Reprod Fertil,1968, 17(3):555-557.
[8]	BIFANO C,LINCK R G.The effect of nonbridging ligands on the rate of electron-transfer reactions[J].J Am Chem Soc,1967, 89(15):3945-3947.
[9]	CHAPEAU C,ENGELHARDT H,KING G J,et al.Alkaline phosphatase activity in the theca of ovarian follicles of the hen throughout follicular development[J].Poult Sci,1996,75(12):1536-1545.
[10]	BAHR J M,WANG S C,HUANG M Y,et al.Steroid concentrations in isolated theca and granulosa layers of preovulatory follicles during the ovulatory cycle of the domestic hen[J].Biol Reprod,1983,29(2):326-334.
[11]	NITTA H,OSAWA Y,BAHR J M.Immunolocalization of steroidogenic cells in small follicles of the chicken ovary:anatomical arrangement and location of steroidogenic cells change during follicular development[J].Domest Anim Endocrinol,1991,8(4):587-594.
[12]	ZADWORNY D,SHIMADA K,ISHIDA H,et al.Gonadotropin-stimulated estradiol production in small ovarian follicles of the hen is suppressed by physiological concentrations of prolactin in vitro[J].Gen Comp Endocrinol,1989,74(3):468-473.
[13]	LI P F,YU X J,XIE J S,et al.Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary[J].Biol Res,2017,50:18.
[14]	LONG L,WU S G,YUAN F,et al.Effects of dietary octacosanol supplementation on laying performance,egg quality,serum hormone levels,and expression of genes related to the reproductive axis in laying hens[J].Poult Sci,2017,96(4):894-903.
[15]	HU S Q,DUGGAVATHI R,ZADWORNY D,et al.Regulatory mechanisms underlying the expression of prolactin receptor in chicken granulosa cells[J].PLoS One,2017,12(1):e0170409.
[16]	XIA W G,FOUAD A M,CHEN W,et al.Estimation of dietary arginine requirements for Longyan laying ducks[J].Poult Sci,2017,96(1):144-150.
[17]	ZHANG Z C,LAI S,WANG Y G,et al.Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen[J].PLoS One,2017,12(6):e0179019.
[18]	PORTER T E,HARGIS B M,SILSBY J L,et al.Enhanced progesterone and testosterone secretion and depressed estradiol secretion in vitro from small white follicle cells of incubating turkey hens[J].Gen Comp Endocrinol,1989,74(3):400-405.
[19]	BRONNEBERG R G G,TAVERNE M A M.Ultrasonography of the female reproductive organs in farmed ostriches (Struthio camelus spp.)[J].Theriogenology,2003,60(4):617-633.
[20]	GOERLICH V C,DIJKSTRA C,GROOTHUIS T G G.No evidence for selective follicle abortion underlying primary sex ratio adjustment in pigeons[J].Behav Ecol Sociobiol,2010,64(4):599-606.
[21]	ALLAHBADIA G N,MORIMOTO Y.Ovarian stimulation protocols[M].New Delhi:Springer,2016.
[22]	ZHANG L N,FENG T,SPICER L.The role of tight junction proteins in ovarian follicular development and ovarian cancer[J].Reproduction,2018,155(4):R183-R198.
[23]	李琴.microRNA对哺乳动物卵泡发育的影响[J].畜牧兽医学报,2018,49(12):2558-2566.
	LI Q.Roles of microRNA on mammalian follicle development[J].Acta Veterinaria et Zootechnica Sinica,2018,49(12):2558-2566.(in Chinese)
[24]	俞萍.鸡早期卵巢发育和原始卵泡形成激素调节的研究[D].杭州:浙江大学,2014.
	YU P.Hormonal regulation of early ovarian development and formation of primordial follicles in the chicken[D].Hangzhou:Zhejiang University,2014.(in Chinese)
[25]	PERRY M M,GILBERT A B.Yolk transport in the ovarian follicle of the hen (Gallus domesticus):lipoprotein-like particles at the periphery of the oocyte in the rapid growth phase[J].J Cell Sci,1979,39:257-272.
[26]	KIM D,LEE J,JOHNSON A L.Vascular endothelial growth factor and angiopoietins during hen ovarian follicle development[J]. Gen Comp Endocrinol,2016,232:25-31.
[27]	LI H,CHIAN R C.Follicular development and oocyte growth[M]//CHIAN R C,NARGUND G,HUANG J Y J.Development of in vitro Maturation for Human Oocytes:Natural and Mild Approaches to Clinical Infertility Treatment.Cham:Springer,2017.
[28]	JOHNSON A L.The avian ovary and follicle development:some comparative and practical insights[J].Turk J Vet Anim Sci,2014, 38:660-669.
[29]	WOODS D C,JOHNSON A L.Regulation of follicle-stimulating hormone-receptor messenger RNA in hen granulosa cells relative to follicle selection[J].Biol Reprod,2005,72(3):643-650.
[30]	KIM D,JOHNSON A L.Differentiation of the granulosa layer from hen prehierarchal follicles associated with follicle-stimulating hormone receptor signaling[J].Mol Reprod Dev,2018,85(8-9):729-737.
[31]	DENG Y,GAN X,CHEN D,et al.Comparison of growth characteristics of in vitro cultured granulosa cells from geese follicles at different developmental stages[J].Biosci Rep,2018,38(2):BSR20171361.
[32]	WOODS D C,SCHOREY J S,JOHNSON A L.Toll-like receptor signaling in hen ovarian granulosa cells is dependent on stage of follicle maturation[J].Reproduction,2009,137(6):987-996.
[33]	KIM D,JOHNSON A L.Vasoactive intestinal peptide promotes differentiation and clock gene expression in granulosa cells from prehierarchal follicles[J].Mol Reprod Dev,2016,83(5):455-463.
[34]	ZHU G Y,CHEN X,MAO Y,et al.Characterization of annexin A2 in chicken follicle development:Evidence for its involvement in angiogenesis[J].Anim Reprod Sci,2015,161:104-111.
[35]	SEGAWA T,TERAMOTO S,OMI K,et al.Changes in estrone and estradiol levels during follicle development:a retrospective large-scale study[J].Reprod Biol Endocrinol,2015,13:54.