畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (8): 1596-1606.doi: 10.11843/j.issn.0366-6964.2019.08.008

• 生物技术与繁殖 • 上一篇    下一篇

基于UPLC-Q-TOF MS代谢组学技术筛选表征西门塔尔种公牛精子活力的候选生物标志物

罗芳1, 郭延生1, 马志远2, 卢旺银2, 李刚2, 陶金忠1*   

  1. 1. 宁夏大学农学院动物科学系, 银川 750021;
    2. 甘肃省家畜繁育改良管理站, 武威 733000
  • 收稿日期:2019-01-08 出版日期:2019-08-23 发布日期:2019-08-19
  • 通讯作者: 陶金忠,主要从事家畜繁殖及繁殖障碍的研究,E-mail:tao_jz@nxu.edu.cn
  • 作者简介:罗芳(1995-),女,甘肃武威人,硕士,主要从事动物生物标识物的筛选,E-mail:1321195999@qq.com
  • 基金资助:
    国家自然科学基金(31661731;31160523)

Selection of Candidate Biomarkers Featuring Sperm Motility in Simmental Bull Based on UPLC-Q-TOF MS Metabolomics

LUO Fang1, GUO Yansheng1, MA Zhiyuan2, LU Wangyin2, LI Gang2, TAO Jinzhong1*   

  1. 1. Department of Animal Science, School of Agricultural, Ningxia University, Yinchuan 750021, China;
    2. Station of Domestic Animal Improving and Management of Gansu Province, Wuwei 733000, China
  • Received:2019-01-08 Online:2019-08-23 Published:2019-08-19

摘要: 旨在筛选表征种公牛精液活力的生物标志物。本试验采集24份西门塔尔种公牛精液,根据精子活力进行分组,其中异常组11个样本,正常组13个;采用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF MS)代谢组学技术探讨了2组精浆代谢轮廓和代谢物的变化趋势。主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)结果显示,异常组精浆代谢轮廓较正常组发生明显变化,6种代谢物水平显著降低(P<0.05),19种代谢物水平显著升高(P<0.05);ROC曲线进一步检测了这些差异代谢物对分类的识别能力,结果显示,仅有6种代谢物(鞘氨醇、环己胺、四氧嘧啶、5'-脱氧腺苷、N6,N6,N6-三甲基-L-赖氨酸、戊酸)对2组精浆具有显著的区分能力。综上表明,精浆中鞘氨醇、环己胺、四氧嘧啶、5'-脱氧腺苷、N6,N6,N6-三甲基-L-赖氨酸、戊酸可作为表征种公牛精液活力的潜在生物标志物,可为研究精液品质的判定技术提供新的方法和思路。

关键词: 西门塔尔种公牛, 精子活力, UPLC-Q-TOF MS, 代谢组学, 生物标志物

Abstract: This study aimed to select biomarkers featuring sperm motility in Simmental bull. Twenty four Simmental bulls semen were collected, eleven of which were viewed as abnormal group, and 13 as normal group according to their sperm motility. UPLC-Q-TOF MS metabolomics was used to study change in metabolic profile and metabolites of seminal plasma in the 2 groups. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) revealed that, compared to the normal group, seminal plasma metabolic profiles obviously altered, six metabolites levels significantly decreased(P<0.05), and 19 metabolites levels significantly increased(P<0.05) in abnormal group. These metabolites were further analyzed by ROC curve to observe their discriminating ability to classification. As a result, only 6 metabolites(including sphingosine, N6, N6, N6-Trimethyl-L-lysine, cyclohexylamine, 5'-Deoxyadenosine, pentadecanoic acid and alloxan) had an obviously distinguishing capacity for seminal plasma in the 2 groups. In conclusion, sphingosine, N6, N6, N6-Trimethyl-L-lysine, cyclohexylamine, 5'-Deoxyadenosine, pentadecanoic acid and alloxan in seminal plasma could be better potential biomarkers to evaluate sperm motility in Simmental bull, which could provide new methods and ideas for the study of semen quality determination technology.

Key words: Simmental bulls, sperm motility, UPLC-Q-TOF MS, metabolomics, biomarkers

中图分类号: