畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (4): 677-687.doi: 10.11843/j.issn.0366-6964.2019.04.001

• 遗传育种 • 上一篇    下一篇

LYRM1基因对脂肪沉积的影响研究

苑洪霞1,2, 骆金红1,2, 冯文武3, 陈祥1,2*   

  1. 1. 贵州大学高原山地动物遗传育种与繁殖教育部重点实验室, 贵州省动物遗传育种与繁殖重点实验室, 贵阳 550025;
    2. 贵州大学动物科学学院, 贵阳 550025;
    3. 贵州省种畜禽种质测定中心, 贵阳 550018
  • 收稿日期:2018-09-03 出版日期:2019-04-23 发布日期:2019-04-23
  • 通讯作者: 陈祥,主要从事动物遗传育种与种质资源创新研究,E-mail:as.xchen2@gzu.edu.cn
  • 作者简介:苑洪霞(1990-),女,山东菏泽人,硕士生,主要从事动物繁殖与生物技术研究,E-mail:771990592@qq.com
  • 基金资助:

    贵州白洗猪选育技术成果推广及产业化(黔科合成果[2017]4412);贵州省农业攻关项目(黔科合NY[2015]3008-1号)

Study on the Effect of LYRM1 Gene on the Fat Deposition of Pig

YUAN Hongxia1,2, LUO Jinhong1,2, FENG Wenwu3, CHEN Xiang1,2*   

  1. 1. Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education, Guizhou University, Guiyang 550025, China;
    2. College of Animal Science, Guizhou University, Guiyang 550025, China;
    3. Guizhou Province Livestock and Poultry Germplasm Determination Center, Guiyang 550018, China
  • Received:2018-09-03 Online:2019-04-23 Published:2019-04-23

摘要:

旨在探究LYRM1对脂肪沉积的影响。本研究采用qRT-PCR法检测LYRM1基因在10月龄白洗猪不同组织中的表达水平;PCR扩增白洗猪LYRM1基因的CDS区,构建pEGFP-N3-LYRM1重组质粒,利用脂质体转染法转染pEGFP-N3-LYRM1重组质粒进入白洗猪皮下脂肪前体细胞,通过检测细胞培养基中三酰甘油浓度,利用血清饥饿法和MTT法检测细胞凋亡水平;qRT-PCR检测LYRM1、PPARγATGLFAS基因的mRNA表达水平。结果显示,LYRM1基因在白洗猪脂肪组织中表达量最高;成功转染pEGFP-N3-LYRM1重组质粒进入白洗猪皮下脂肪前体细胞,转染试验组培养基中三酰甘油浓度大于空白对照组,试验组与空白对照组的细胞凋亡速率一致,未见显著差异;试验组LYRM1、PPARγATGLFAS基因的mRNA表达量极显著高于对照组(P<0.01)。综上表明,LYRM1基因超表达可提高三酰甘油的浓度,促进脂肪沉积,提高脂肪沉积相关基因mRNA的表达水平,间接提高脂肪沉积水平。因此认为,LYRM1基因可作为探究影响脂肪沉积的候选基因,为研究脂肪沉积机制提供理论基础。

关键词: LYRM1, 三酰甘油, 细胞凋亡, 脂肪沉积

Abstract:

The purpose of this study was to explore the effect of pig LYRM1 gene on the fat deposition. The expression levels of LYRM1 gene in different tissues of Baixi pigs aged 10 months were first detected by qRT-PCR. CDS of LYRM1 gene was amplified by PCR. The recombinant eukaryotic expression vector pEGFP-N3-LYRM1 was constructed and then transfected into progenitor cells of subcutaneous adipose tissue of Baixi pig. The cell apoptosis of transfected cells was analyzed by triacylglycerol concentration detection, serum starvation method and MTT assay. The mRNA expression levels of LYRM1, PPARγ, ATGL and FAS genes were detected by qRT-PCR. The results showed that LYRM1 gene had the highest expression level in adipose tissues of Baixi pigs. The concentration of triacylglycerol in progenitor cells of subcutaneous adipose tissue transfected with pEGFP-N3-LYRM1 was higher than that in the control group. The apoptosis rates of the experimental group cells and the control group cells were similar and no significant difference was found. The mRNA expression levels of LYRM1, PPARγ, ATGL and FAS genes in the experimental group were significantly higher than that in the control group (P<0.01). In conclusion, overexpression of LYRM1 gene can increase the concentration of triacylglycerol, promote fat deposition, enhance the mRNA expression level of genes related to fat deposition, and indirectly increase the level of fat deposition. Therefore, LYRM1 gene can be regarded as a candidate gene to study the fat deposition, which provide a theoretical basis for investigating the mechanism of fat deposition.

Key words: LYRM1, triacylglycerol, apoptosis, fat deposition

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